View source for 1ghh

[[Image:1ghh.gif|left|200px]]<br /><applet load="1ghh" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1ghh" />
'''SOLUTION STRUCTURE OF DINI'''<br />

==Overview==
The Escherichia coli RecA protein triggers both DNA repair and mutagenesis, in a process known as the SOS response. The 81-residue E. coli protein, DinI inhibits activity of RecA in vivo. The solution structure of DinI has, been determined by multidimensional triple resonance NMR spectroscopy, using restraints derived from two sets of residual dipolar couplings, obtained in bicelle and phage media, supplemented with J couplings and a, moderate number of NOE restraints. DinI has an alpha/beta fold comprised, of a three-stranded beta-sheet and two alpha-helices. The beta-sheet, topology is unusual: the central strand is flanked by a parallel and an, antiparallel strand and the sheet is remarkably flat. The structure of, DinI shows that six negatively charged Glu and Asp residues on DinI's, kinked C-terminal alpha-helix form an extended, negatively charged ridge., We propose that this ridge mimics the electrostatic character of the DNA, phospodiester backbone, thereby enabling DinI to compete with, single-stranded DNA for RecA binding. Biochemical data confirm that DinI, is able to displace ssDNA from RecA.

==About this Structure==
1GHH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure superseeds the now removed PDB entry 1F0A. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GHH OCA]. 

==Reference==
Solution structure of DinI provides insight into its mode of RecA inactivation., Ramirez BE, Voloshin ON, Camerini-Otero RD, Bax A, Protein Sci. 2000 Nov;9(11):2161-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11152126 11152126]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Bax, A.]]
[[Category: Camerini-Otero, R.D.]]
[[Category: Ramirez, B.E.]]
[[Category: Voloshin, O.N.]]
[[Category: bicelle]]
[[Category: dini]]
[[Category: dipolar coupling]]
[[Category: liquid crystal]]
[[Category: nmr]]
[[Category: pf1]]
[[Category: reca]]

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