View source for 1g76

[[Image:1g76.gif|left|200px]]<br /><applet load="1g76" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1g76, resolution 2.20&Aring;" />
'''X-RAY STRUCTURE OF ESCHERICHIA COLI PYRIDOXINE 5'-PHOSPHATE OXIDASE COMPLEXED WITH PYRIDOXAL 5'-PHOSPHATE AT 2.0 A RESOLUTION'''<br />

==Overview==
Escherichia coli pyridoxine 5'-phosphate oxidase catalyzes the terminal, step in the biosynthesis of pyridoxal 5'-phosphate by the FMN oxidation of, pyridoxine 5'-phosphate forming FMNH(2) and H(2)O(2). Recent studies have, shown that in addition to the active site, pyridoxine 5'-phosphate oxidase, contains a non-catalytic site that binds pyridoxal 5'-phosphate tightly., The crystal structure of pyridoxine 5'-phosphate oxidase from E. coli with, one or two molecules of pyridoxal 5'-phosphate bound to each monomer has, been determined to 2.0 A resolution. One of the pyridoxal 5'-phosphate, molecules is clearly bound at the active site with the aldehyde at C4' of, pyridoxal 5'-phosphate near N5 of the bound FMN. A protein conformational, change has occurred that partially closes the active site. The orientation, of the bound pyridoxal 5'-phosphate suggests that the enzyme catalyzes a, hydride ion transfer between C4' of pyridoxal 5'-phosphate and N5 of FMN., When the crystals are soaked with excess pyridoxal 5'-phosphate an, additional molecule of this cofactor is also bound about 11 A from the, active site. A possible tunnel exists between the two sites so that, pyridoxal 5'-phosphate formed at the active site may transfer to the, non-catalytic site without passing though the solvent.

==About this Structure==
1G76 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with PO4, FMN and PLP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pyridoxal_5'-phosphate_synthase Pyridoxal 5'-phosphate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.5 1.4.3.5] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1G76 OCA]. 

==Reference==
X-ray structure of Escherichia coli pyridoxine 5'-phosphate oxidase complexed with pyridoxal 5'-phosphate at 2.0 A resolution., Safo MK, Musayev FN, di Salvo ML, Schirch V, J Mol Biol. 2001 Jul 20;310(4):817-26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11453690 11453690]
[[Category: Escherichia coli]]
[[Category: Pyridoxal 5'-phosphate synthase]]
[[Category: Single protein]]
[[Category: Musayev, F.N.]]
[[Category: Safo, M.K.]]
[[Category: Salvo, M.L.di.]]
[[Category: Schirch, V.]]
[[Category: FMN]]
[[Category: PLP]]
[[Category: PO4]]
[[Category: fmn complex]]
[[Category: plp complex]]
[[Category: pyridoxine 5'-phosphate]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:46:29 2007''