View source for 1g72

[[Image:1g72.jpg|left|200px]]<br /><applet load="1g72" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1g72, resolution 1.90&Aring;" />
'''CATALYTIC MECHANISM OF QUINOPROTEIN METHANOL DEHYDROGENASE: A THEORETICAL AND X-RAY CRYSTALLOGRAPHIC INVESTIGATION'''<br />

==Overview==
The catalytic mechanism of the reductive half reaction of the quinoprotein, methanol dehydrogenase (MDH) is believed to proceed either through a, hemiketal intermediate or by direct transfer of a hydride ion from the, substrate methyl group to the cofactor, pyrroloquinoline quinone (PQQ). A, crystal structure of the enzyme-substrate complex of a similar, quinoprotein, glucose dehydrogenase, has recently been reported that, strongly favors the hydride transfer mechanism in that enzyme. A, theoretical analysis and an improved refinement of the 1.9-A resolution, crystal structure of MDH from Methylophilus methylotrophus W3A1 in the, presence of methanol, reported earlier, indicates that the observed, tetrahedral configuration of the C-5 atom of PQQ in that study represents, the C-5-reduced form of the cofactor and lends support for a hydride, transfer mechanism for MDH.

==About this Structure==
1G72 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Methylophilus_methylotrophus Methylophilus methylotrophus] with CA and PQQ as [http://en.wikipedia.org/wiki/ligands ligands]. This structure superseeds the now removed PDB entry 1B2N. Active as [http://en.wikipedia.org/wiki/Alcohol_dehydrogenase_(acceptor) Alcohol dehydrogenase (acceptor)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.99.8 1.1.99.8] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1G72 OCA]. 

==Reference==
Catalytic mechanism of quinoprotein methanol dehydrogenase: A theoretical and x-ray crystallographic investigation., Zheng YJ, Xia Zx, Chen Zw, Mathews FS, Bruice TC, Proc Natl Acad Sci U S A. 2001 Jan 16;98(2):432-4. Epub 2001 Jan 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11149955 11149955]
[[Category: Alcohol dehydrogenase (acceptor)]]
[[Category: Methylophilus methylotrophus]]
[[Category: Protein complex]]
[[Category: Bruice, T.C.]]
[[Category: Chen, Z.]]
[[Category: Mathews, F.S.]]
[[Category: Xia, Z.]]
[[Category: Zheng, Y.]]
[[Category: CA]]
[[Category: PQQ]]
[[Category: quinoprotein]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:46:20 2007''