View source for 1ofd

[[Image:1ofd.gif|left|200px]]<br />
<applet load="1ofd" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1ofd, resolution 2.0&Aring;" />
'''GLUTAMATE SYNTHASE FROM SYNECHOCYSTIS SP IN COMPLEX WITH 2-OXOGLUTARATE AT 2.0 ANGSTROM RESOLUTION'''<br />

==Overview==
Glutamate synthases (GltS) are crucial enzymes in ammonia assimilation in, plants and bacteria, where they catalyze the formation of two molecules of, L-glutamate from L-glutamine and 2-oxoglutarate. The plant-type, ferredoxin-dependent GltS and the functionally homologous alpha subunit of, the bacterial NADPH-dependent GltS are complex four-domain monomeric, enzymes of 140-165 kDa belonging to the NH(2)-terminal nucleophile family, of amidotransferases. The enzymes function through the channeling of, ammonia from the N-terminal amidotransferase domain to the FMN-binding, domain. Here, we report the X-ray structure of the Synechocystis, ferredoxin-dependent GltS with the substrate 2-oxoglutarate and the, covalent inhibitor 5-oxo-L-norleucine bound in their physically distinct, active ... [[http://ispc.weizmann.ac.il/pmbin/getpm?12818206 (full description)]]

==About this Structure==
1OFD is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.]] with FMN, F3S and AKG as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.7.1 1.4.7.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OFD OCA]]. 

==Reference==
The active conformation of glutamate synthase and its binding to ferredoxin., van den Heuvel RH, Svergun DI, Petoukhov MV, Coda A, Curti B, Ravasio S, Vanoni MA, Mattevi A, J Mol Biol. 2003 Jun 27;330(1):113-28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12818206 12818206]
[[Category: Single protein]]
[[Category: Synechocystis sp.]]
[[Category: Heuvel, R.H.H.Van.Den.]]
[[Category: Mattevi, A.]]
[[Category: AKG]]
[[Category: F3S]]
[[Category: FMN]]
[[Category: amidotransferase]]
[[Category: complex enzyme]]
[[Category: fad]]
[[Category: fd-gogat]]
[[Category: flavoprotein]]
[[Category: fmn]]
[[Category: iron-sulphur]]
[[Category: oxidoreductase]]
[[Category: substrate channeling]]

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