View source for 1eam

[[Image:1eam.gif|left|200px]]<br /><applet load="1eam" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1eam, resolution 2.0&Aring;" />
'''VACCINIA METHYLTRANSFERASE VP39 MUTANT (EC: 2.7.7.19)'''<br />

==Overview==
We have determined, by high resolution x-ray analysis, 10 structures, comprising the mRNA cap-specific methyltransferase VP39 or specific, mutants thereof in the presence of methylated nucleobase analogs, (N1-methyladenine, N3-methyladenine, N1-methylcytosine, N3-methylcytosine), and their unmethylated counterparts, or nucleoside N7-methylguanosine., Together with solution affinity studies and previous crystallographic data, for N7-methylguanosine and its phosphorylated derivatives, these data, demonstrate that only methylated, positively charged bases are bound, indicating that their enhanced stacking with two aromatic side chains of, VP39 (Tyr 22 and Phe 180) plays a dominant role in cap recognition. Four, key features characterize this stacking interaction: (i) near perfect, parallel alignment between the sandwiched methylated bases and aromatic, side chains, (ii) substantial areas of overlap in the two-stacked rings, (iii) a 3.4-A interplanar spacing within the overlapping region, and (iv), positive charge in the heterocyclic nucleobase.

==About this Structure==
1EAM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus] with SAH as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Polynucleotide_adenylyltransferase Polynucleotide adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.19 2.7.7.19] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EAM OCA]. 

==Reference==
mRNA cap recognition: dominant role of enhanced stacking interactions between methylated bases and protein aromatic side chains., Hu G, Gershon PD, Hodel AE, Quiocho FA, Proc Natl Acad Sci U S A. 1999 Jun 22;96(13):7149-54. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10377383 10377383]
[[Category: Polynucleotide adenylyltransferase]]
[[Category: Single protein]]
[[Category: Vaccinia virus]]
[[Category: Gershon, P.D.]]
[[Category: Hodel, A.E.]]
[[Category: Hu, G.]]
[[Category: Quiocho, F.A.]]
[[Category: SAH]]
[[Category: methylated guanosine]]
[[Category: methyltransferase mutant e233a]]
[[Category: mrna processing]]
[[Category: poly (a) polymerase]]
[[Category: transcription]]
[[Category: vaccinia]]

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