View source for 1b10

[[Image:1b10.gif|left|200px]]<br /><applet load="1b10" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1b10" />
'''SOLUTION NMR STRUCTURE OF RECOMBINANT SYRIAN HAMSTER PRION PROTEIN RPRP(90-231) , 25 STRUCTURES'''<br />

==Overview==
The scrapie prion protein (PrPSc) is the major, and possibly the only, component of the infectious prion; it is generated from the cellular, isoform (PrPC) by a conformational change. N-terminal truncation of PrPSc, by limited proteolysis produces a protein of approximately 142 residues, designated PrP 27-30, which retains infectivity. A recombinant protein, (rPrP) corresponding to Syrian hamster PrP 27-30 was expressed in, Escherichia coli and purified. After refolding rPrP into an alpha-helical, form resembling PrPC, the structure was solved by multidimensional, heteronuclear NMR, revealing many structural features of rPrP that were, not found in two shorter PrP fragments studied previously. Extensive, side-chain interactions for residues 113-125 characterize a hydrophobic, cluster, which packs against an irregular beta-sheet, whereas residues, 90-112 exhibit little defined structure. Although identifiable secondary, structure is largely lacking in the N terminus of rPrP, paradoxically this, N terminus increases the amount of secondary structure in the remainder of, rPrP. The surface of a long helix (residues 200-227) and a structured loop, (residues 165-171) form a discontinuous epitope for binding of a protein, that facilitates PrPSc formation. Polymorphic residues within this epitope, seem to modulate susceptibility of sheep and humans to prion disease., Conformational heterogeneity of rPrP at the N terminus may be key to the, transformation of PrPC into PrPSc, whereas the discontinuous epitope near, the C terminus controls this transition.

==About this Structure==
1B10 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mesocricetus_auratus Mesocricetus auratus]. This structure superseeds the now removed PDB entry 2PRP. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1B10 OCA]. 

==Reference==
Solution structure of a 142-residue recombinant prion protein corresponding to the infectious fragment of the scrapie isoform., James TL, Liu H, Ulyanov NB, Farr-Jones S, Zhang H, Donne DG, Kaneko K, Groth D, Mehlhorn I, Prusiner SB, Cohen FE, Proc Natl Acad Sci U S A. 1997 Sep 16;94(19):10086-91. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9294167 9294167]
[[Category: Mesocricetus auratus]]
[[Category: Single protein]]
[[Category: Farr-Jones, S.]]
[[Category: James, T.L.]]
[[Category: Liu, H.]]
[[Category: Ulyanov, N.B.]]
[[Category: brain]]
[[Category: glycoprotein]]
[[Category: prion]]
[[Category: scrapie]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:16:16 2007''