View source for 2qsc

[[Image:2qsc.jpg|left|200px]]

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{{STRUCTURE_2qsc|  PDB=2qsc  |  SCENE=  }} 

'''Crystal structure analysis of anti-HIV-1 V3-Fab F425-B4e8 in complex with a V3-peptide'''


==Overview==
F425-B4e8 (B4e8) is a monoclonal antibody isolated from a human immunodeficiency virus type 1 (HIV-1)-infected individual that recognizes the V3 variable loop on the gp120 subunit of the viral envelope spike. B4e8 neutralizes a subset of HIV-1 primary isolates from subtypes B, C and D, which places this antibody among the very few human anti-V3 antibodies with notable cross-neutralizing activity. Here, the crystal structure of the B4e8 Fab' fragment in complex with a 24-mer V3 peptide (RP142) at 2.8 A resolution is described. The complex structure reveals that the antibody recognizes a novel V3 loop conformation, featuring a five-residue alpha-turn around the conserved GPGRA apex of the beta-hairpin loop. In agreement with previous mutagenesis analyses, the Fab' interacts primarily with V3 through side-chain contacts with just two residues, Ile(P309) and Arg(P315), while the remaining contacts are to the main chain. The structure helps explain how B4e8 can tolerate a certain degree of sequence variation within V3 and, hence, is able to neutralize an appreciable number of different HIV-1 isolates.

==About this Structure==
2QSC is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QSC OCA]. 

==Reference==
Structure of antibody F425-B4e8 in complex with a V3 peptide reveals a new binding mode for HIV-1 neutralization., Bell CH, Pantophlet R, Schiefner A, Cavacini LA, Stanfield RL, Burton DR, Wilson IA, J Mol Biol. 2008 Jan 25;375(4):969-78. Epub 2007 Nov 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18068724 18068724]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Bell, C H.]]
[[Category: Schiefner, A.]]
[[Category: Stanfield, R L.]]
[[Category: Wilson, I A.]]
[[Category: Aid]]
[[Category: Apoptosis]]
[[Category: Envelope protein]]
[[Category: Fab-peptide complex]]
[[Category: Fusion protein]]
[[Category: Glycoprotein]]
[[Category: Gp120]]
[[Category: Hiv-1]]
[[Category: Host-virus interaction]]
[[Category: Immune system]]
[[Category: Immunoglobulin fold]]
[[Category: Membrane]]
[[Category: Transmembrane]]
[[Category: V3 loop]]
[[Category: Viral immunoevasion]]
[[Category: Virion]]
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