View source for 2npx

[[Image:2npx.jpg|left|200px]]

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{{STRUCTURE_2npx|  PDB=2npx  |  SCENE=  }} 

'''NADH BINDING SITE AND CATALYSIS OF NADH PEROXIDASE'''


==Overview==
The structure of the complex between cofactor NADH and the enzyme NADH peroxidase from Streptococcus faecalis 10C1 (Enterococcus faecalis) has been determined by crystal soaking, X-ray data collection, model building of NADH and refinement at 0.24-nm resolution based on the known enzyme structure [Stehle, T., Ahmed, S. A., Claiborne, A. &amp; Schulz, G. E. (1991) J. Mol. Biol. 221, 1325-1344]. Apart from NADH, the catalytic center of the enzyme contains FAD and a cysteine that shuttles between thiolate and sulfenic acid states. Unfortunately, this cysteine was irreversibly oxidized to a cysteine sulfonic acid in the established enzyme structure. Based on the geometry of the catalytic center, we discuss the stabilization of the oxidation-sensitive sulfenic acid and propose a reaction mechanism.

==About this Structure==
2NPX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NPX OCA]. 

==Reference==
NADH binding site and catalysis of NADH peroxidase., Stehle T, Claiborne A, Schulz GE, Eur J Biochem. 1993 Jan 15;211(1-2):221-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8425532 8425532]
[[Category: Enterococcus faecalis]]
[[Category: NADH peroxidase]]
[[Category: Single protein]]
[[Category: Claiborne, A.]]
[[Category: Schulz, G E.]]
[[Category: Stehle, T.]]
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