View source for 3fib

[[Image:3fib.gif|left|200px]]<br />
<applet load="3fib" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="3fib, resolution 2.1&Aring;" />
'''RECOMBINANT HUMAN GAMMA-FIBRINOGEN CARBOXYL TERMINAL FRAGMENT (RESIDUES 143-411) BOUND TO CALCIUM AT PH 6.0: A FURTHER REFINEMENT OF PDB ENTRY 1FIB, AND DIFFERS FROM 1FIB BY THE MODELLING OF A CIS PEPTIDE BOND BETWEEN RESIDUES K338 AND C339'''<br />

==Overview==
After vascular injury, a cascade of serine protease activations leads to, the conversion of the soluble fibrinogen molecule into fibrin. The fibrin, monomers then polymerize spontaneously and noncovalently to form a fibrin, gel. The primary interaction of this polymerization reaction is between, the newly exposed N-terminal Gly-Pro-Arg sequence of the alpha chain of, one fibrin molecule and the C-terminal region of a gamma chain of an, adjacent fibrin(ogen) molecule. In this report, the polymerization pocket, has been identified by determining the crystal structure of a 30-kDa, C-terminal fragment of the fibrin(ogen) gamma chain complexed with the, peptide Gly-Pro-Arg-Pro. This peptide mimics the N terminus of the alpha, chain of fibrin. The conformational change in the protein upon binding the, peptide is subtle, with electrostatic interactions primarily mediating the, association. This is consistent with biophysical experiments carried out, over the last 50 years on this fundamental polymerization reaction.

==Disease==
Known diseases associated with this structure: Dysfibrinogenemia, gamma type OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=134850 134850]], Hypofibrinogenemia, gamma type OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=134850 134850]], Thrombophilia, dysfibrinogenemic OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=134850 134850]]

==About this Structure==
3FIB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=3FIB OCA]. 

==Reference==
The primary fibrin polymerization pocket: three-dimensional structure of a 30-kDa C-terminal gamma chain fragment complexed with the peptide Gly-Pro-Arg-Pro., Pratt KP, Cote HC, Chung DW, Stenkamp RE, Davie EW, Proc Natl Acad Sci U S A. 1997 Jul 8;94(14):7176-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9207064 9207064]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Chung, D.W.]]
[[Category: Cote, H.C.F.]]
[[Category: Davie, E.W.]]
[[Category: Pratt, K.P.]]
[[Category: Stenkamp, R.E.]]
[[Category: CA]]
[[Category: blood coagulation]]
[[Category: cis peptide bonds]]
[[Category: fibrin polymerization]]
[[Category: fibrinogen]]

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