View source for 2bax

[[Image:2bax.gif|left|200px]]

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{{STRUCTURE_2bax|  PDB=2bax  |  SCENE=  }} 

'''Atomic Resolution Structure of the Double Mutant (K53,56M) of Bovine Pancreatic Phospholipase A2'''


==Overview==
The enzyme phospholipase A2 catalyzes the hydrolysis of the sn-2 acyl chain of phospholipids, forming fatty acids and lysophospholipids. The crystal structure of a triple mutant (K53,56,121M) of bovine pancreatic phospholipase A2 in which the lysine residues at positions 53, 56 and 121 are replaced recombinantly by methionines has been determined at atomic resolution (0.97 A). The crystal is monoclinic (space group P2), with unit-cell parameters a = 36.934, b = 23.863, c = 65.931 A, beta = 101.47 degrees. The structure was solved by molecular replacement and has been refined to a final R factor of 10.6% (Rfree = 13.4%) using 63,926 unique reflections. The final protein model consists of 123 amino-acid residues, two calcium ions, one chloride ion, 243 water molecules and six 2-methyl-2,4-pentanediol molecules. The surface-loop residues 60-70 are ordered and have clear electron density.

==About this Structure==
2BAX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BAX OCA]. 

==Reference==
Atomic resolution (0.97 A) structure of the triple mutant (K53,56,121M) of bovine pancreatic phospholipase A2., Sekar K, Rajakannan V, Gayathri D, Velmurugan D, Poi MJ, Dauter M, Dauter Z, Tsai MD, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt 1):3-7., Epub 2004 Sep 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16508077 16508077]
[[Category: Bos taurus]]
[[Category: Single protein]]
[[Category: Dauter, M.]]
[[Category: Dauter, Z.]]
[[Category: Sekar, K.]]
[[Category: Tsai, M D.]]
[[Category: Velmurugan, D.]]
[[Category: Yogavel, M.]]
[[Category: Alpha helix]]
[[Category: Beta sheet]]
[[Category: Phospholipase a2]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 20:03:22 2008''