View source for 2aak

[[Image:2aak.jpg|left|200px]]

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{{STRUCTURE_2aak|  PDB=2aak  |  SCENE=  }} 

'''UBIQUITIN CONJUGATING ENZYME FROM ARABIDOPSIS THALIANA'''


==Overview==
The x-ray crystal structure of a recombinant ubiquitin-conjugating enzyme (E2) encoded by the UBC1 gene of the plant Arabidopsis thaliana has been determined with the use of multiple isomorphous replacement techniques and refined at 2.4-A resolution by simulated annealing and restrained least-squares. This E2 is an alpha/beta protein, with four alpha-helices and a four-stranded antiparallel beta-sheet. The NH2 and COOH termini, which may be important for interaction with other enzymes and substrates in the ubiquitin-conjugation pathway, are on the opposite side of the molecule from the cysteine residue that binds to the COOH terminus of ubiquitin. This structure should now allow for the rational analysis of E2 function by in vitro mutagenesis and facilitate the effective design of E2s with unique specificities or catalytic functions.

==About this Structure==
2AAK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1aak 1aak]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AAK OCA]. 

==Reference==
Three-dimensional structure of a ubiquitin-conjugating enzyme (E2)., Cook WJ, Jeffrey LC, Sullivan ML, Vierstra RD, J Biol Chem. 1992 Jul 25;267(21):15116-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1321826 1321826]
[[Category: Arabidopsis thaliana]]
[[Category: Single protein]]
[[Category: Ubiquitin--protein ligase]]
[[Category: Cook, W J.]]
[[Category: Jeffrey, L C.]]
[[Category: Sullivan, M L.]]
[[Category: Vierstra, R D.]]
[[Category: Ligase]]
[[Category: Ubiquitin conjugation]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 18:49:06 2008''