View source for 1wac

[[Image:1wac.gif|left|200px]]

<!--
The line below this paragraph, containing "STRUCTURE_1wac", creates the "Structure Box" on the page.
You may change the PDB parameter (which sets the PDB file loaded into the applet) 
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
or leave the SCENE parameter empty for the default display.
-->
{{STRUCTURE_1wac|  PDB=1wac  |  SCENE=  }} 

'''BACK-PRIMING MODE OF PHI6 RNA-DEPENDENT RNA POLYMERASE'''


==Overview==
The RNA-dependent RNA polymerase of the double-stranded RNA bacteriophage phi6 is capable of primer-independent initiation, as are many RNA polymerases. The structure of this polymerase revealed an initiation platform, composed of a loop in the C-terminal domain (QYKW, aa 629-632), that was essential for de novo initiation. A similar element has been identified in hepatitis C virus RNA-dependent RNA polymerase. Biochemical studies have addressed the role of this platform, revealing that a mutant version can utilize a back-priming initiation mechanism, where the 3' terminus of the template adopts a hairpin-like conformation. Here, the mechanism of back-primed initiation is studied further by biochemical and structural methods.

==About this Structure==
1WAC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_phi6 Pseudomonas phage phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAC OCA]. 

==Reference==
Back-priming mode of phi6 RNA-dependent RNA polymerase., Laurila MR, Salgado PS, Stuart DI, Grimes JM, Bamford DH, J Gen Virol. 2005 Feb;86(Pt 2):521-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15659773 15659773]
[[Category: Pseudomonas phage phi6]]
[[Category: Single protein]]
[[Category: Bamford, D H.]]
[[Category: Grimes, J M.]]
[[Category: Laurila, M R.L.]]
[[Category: Salgado, P S.]]
[[Category: Stuart, D I.]]
[[Category: Phi6 rna-dependent rna polymerase]]
[[Category: Transcription]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 13:22:49 2008''