View source for 1vas

[[Image:1vas.gif|left|200px]]

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{{STRUCTURE_1vas|  PDB=1vas  |  SCENE=  }} 

'''ATOMIC MODEL OF A PYRIMIDINE DIMER SPECIFIC EXCISION REPAIR ENZYME COMPLEXED WITH A DNA SUBSTRATE: STRUCTURAL BASIS FOR DAMAGED DNA RECOGNITION'''


==Overview==
T4 endonuclease V is a DNA repair enzyme from bacteriophage T4 that catalyzes the first reaction step of the pyrimidine dimer-specific base excision repair pathway. The crystal structure of this enzyme complexed with a duplex DNA substrate, containing a thymine dimer, has been determined at 2.75 A resolution. The atomic structure of the complex reveals the unique conformation of the DNA duplex, which exhibits a sharp kink with a 60 degree inclination at the central thymine dimer. The adenine base complementary to the 5' side of the thymine dimer is completely flipped out of the DNA duplex and trapped in a cavity on the protein surface. These structural features allow an understanding of the catalytic mechanism and implicate a general mechanism of how other repair enzymes recognize damaged DNA duplexes.

==About this Structure==
1VAS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. The following page contains interesting information on the relation of 1VAS with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb91_1.html Thymine Dimers]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VAS OCA]. 

==Reference==
Atomic model of a pyrimidine dimer excision repair enzyme complexed with a DNA substrate: structural basis for damaged DNA recognition., Vassylyev DG, Kashiwagi T, Mikami Y, Ariyoshi M, Iwai S, Ohtsuka E, Morikawa K, Cell. 1995 Dec 1;83(5):773-82. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8521494 8521494]
[[Category: Enterobacteria phage t4]]
[[Category: Single protein]]
[[Category: Thymine Dimers]]
[[Category: Ariyoshi, M.]]
[[Category: Iwai, S.]]
[[Category: Kashiwagi, T.]]
[[Category: Mikami, Y.]]
[[Category: Morikawa, K.]]
[[Category: Ohtsuka, E.]]
[[Category: Vassylyev, D G.]]
[[Category: Double helix]]
[[Category: Protein-dna complex]]
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