View source for 1qme

[[Image:1qme.jpg|left|200px]]

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{{STRUCTURE_1qme|  PDB=1qme  |  SCENE=  }} 

'''PENICILLIN-BINDING PROTEIN 2X (PBP-2X)'''


==Overview==
Penicillin-binding proteins (PBPs), the primary targets for beta-lactam antibiotics, are periplasmic membrane-attached proteins responsible for the construction and maintenance of the bacterial cell wall. Bacteria have developed several mechanisms of resistance, one of which is the mutation of the target enzymes to reduce their affinity for beta-lactam antibiotics. Here, we describe the structure of PBP2x from Streptococcus pneumoniae determined to 2.4 A. In addition, we also describe the PBP2x structure in complex with cefuroxime, a therapeutically relevant antibiotic, at 2.8 A. Surprisingly, two antibiotic molecules are observed: one as a covalent complex with the active-site serine residue, and a second one between the C-terminal and the transpeptidase domains. The structure of PBP2x reveals an active site similar to those of the class A beta-lactamases, albeit with an absence of unambiguous deacylation machinery. The structure highlights a few amino acid residues, namely Thr338, Thr550 and Gln552, which are directly related to the resistance phenomenon.

==About this Structure==
1QME is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QME OCA]. 

==Reference==
The crystal structure of the penicillin-binding protein 2x from Streptococcus pneumoniae and its acyl-enzyme form: implication in drug resistance., Gordon E, Mouz N, Duee E, Dideberg O, J Mol Biol. 2000 Jun 2;299(2):477-85. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10860753 10860753]
[[Category: Single protein]]
[[Category: Streptococcus pneumoniae]]
[[Category: Dideberg, O.]]
[[Category: Duee, E.]]
[[Category: Gordon, E J.]]
[[Category: Mouz, N.]]
[[Category: Cell wall]]
[[Category: Peptidoglycan synthesis]]
[[Category: Resistance]]
[[Category: Transmembrane]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 06:26:57 2008''