View source for 1qb4

[[Image:1qb4.gif|left|200px]]

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{{STRUCTURE_1qb4|  PDB=1qb4  |  SCENE=  }} 

'''CRYSTAL STRUCTURE OF MN(2+)-BOUND PHOSPHOENOLPYRUVATE CARBOXYLASE'''


==Overview==
We have determined the crystal structure of Mn2+-bound Escherichia coli phosphoenolpyruvate carboxylase (PEPC) using X-ray diffraction at 2.6 A resolution, and specified the location of enzyme-bound Mn2+, which is essential for catalytic activity. The electron density map reveals that Mn2+ is bound to the side chain oxygens of Glu-506 and Asp-543, and located at the top of the alpha/beta barrel in PEPC. The coordination sphere of Mn2+ observed in E. coli PEPC is similar to that of Mn2+ found in the pyruvate kinase structure. The model study of Mn2+-bound PEPC complexed with phosphoenolpyruvate (PEP) reveals that the side chains of Arg-396, Arg-581 and Arg-713 could interact with PEP.

==About this Structure==
1QB4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QB4 OCA]. 

==Reference==
Plausible phosphoenolpyruvate binding site revealed by 2.6 A structure of Mn2+-bound phosphoenolpyruvate carboxylase from Escherichia coli., Matsumura H, Terada M, Shirakata S, Inoue T, Yoshinaga T, Izui K, Kai Y, FEBS Lett. 1999 Sep 17;458(2):93-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10481043 10481043]
[[Category: Escherichia coli]]
[[Category: Phosphoenolpyruvate carboxylase]]
[[Category: Single protein]]
[[Category: Inoue, T.]]
[[Category: Izui, K.]]
[[Category: Kai, Y.]]
[[Category: Matsumura, H.]]
[[Category: Shirakata, S.]]
[[Category: Terada, M.]]
[[Category: Yoshinaga, T.]]
[[Category: Alpha beta barrel]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 06:05:22 2008''