View source for 1njg

[[Image:1njg.gif|left|200px]]

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{{STRUCTURE_1njg|  PDB=1njg  |  SCENE=  }} 

'''Nucleotide-free form of an Isolated E. coli Clamp Loader Gamma Subunit'''


==Overview==
Sliding clamps are loaded onto DNA by ATP-driven clamp loader complexes. The structure of the E. coli clamp loader in a nucleotide-free state has been determined previously. We now report crystal structures of a truncated form of the isolated gamma-ATPase subunit, gamma(1-243), of the E. coli clamp loader, in nucleotide-free and bound forms. The gamma subunit adopts a defined conformation when empty, in which the nucleotide binding site is blocked. The binding of either ATPgammaS or ADP, which are shown to bind with equal affinity to gamma(1-243), induces a change in the relative orientation of the two domains such that nucleotides can be accommodated. This change would break one of the gamma:gamma interfaces seen in the empty clamp loader complex, and may represent one step in the activation process.

==About this Structure==
1NJG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NJG OCA]. 

==Reference==
Nucleotide-induced conformational changes in an isolated Escherichia coli DNA polymerase III clamp loader subunit., Podobnik M, Weitze TF, O'Donnell M, Kuriyan J, Structure. 2003 Mar;11(3):253-63. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12623013 12623013]
[[Category: DNA-directed DNA polymerase]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Donnell, M O.]]
[[Category: Kuriyan, J.]]
[[Category: Podobnik, M.]]
[[Category: Weitze, T F.]]
[[Category: Aaa+ atpase domain]]
[[Category: Rossman-like fold]]
[[Category: Sensor 1]]
[[Category: Sensor 2]]
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