View source for 5kpw


==Structure of RelA bound to ribosome in presence of A/R tRNA (Structure III)==
<SX load='5kpw' size='340' side='right' viewer='molstar' caption='[[5kpw]], [[Resolution|resolution]] 3.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5kpw]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KPW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KPW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kpw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kpw OCA], [https://pdbe.org/5kpw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kpw RCSB], [https://www.ebi.ac.uk/pdbsum/5kpw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kpw ProSAT]</span></td></tr>
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== Function ==
[https://www.uniprot.org/uniprot/RL2_ECOLI RL2_ECOLI] One of the primary rRNA binding proteins. Located near the base of the L1 stalk, it is probably also mobile. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is highly controversial.[HAMAP-Rule:MF_01320_B]  In the E.coli 70S ribosome in the initiation state it has been modeled to make several contacts with the 16S rRNA (forming bridge B7b, PubMed:12809609); these contacts are broken in the model with bound EF-G.[HAMAP-Rule:MF_01320_B]
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== Publication Abstract from PubMed ==
Stringent response is a conserved bacterial stress response underlying virulence and antibiotic resistance. RelA/SpoT-homolog proteins synthesize transcriptional modulators (p)ppGpp, allowing bacteria to adapt to stress. RelA is activated during amino-acid starvation, when cognate deacyl-tRNA binds to the ribosomal A (aminoacyl-tRNA) site. We report four cryo-EM structures of E. coli RelA bound to the 70S ribosome, in the absence and presence of deacyl-tRNA accommodating in the 30S A site. The boomerang-shaped RelA with a wingspan of more than 100 A wraps around the A/R (30S A-site/RelA-bound) tRNA. The CCA end of the A/R tRNA pins the central TGS domain against the 30S subunit, presenting the (p)ppGpp-synthetase domain near the 30S spur. The ribosome and A/R tRNA are captured in three conformations, revealing hitherto elusive states of tRNA engagement with the ribosomal decoding center. Decoding-center rearrangements are coupled with the step-wise 30S-subunit 'closure', providing insights into the dynamics of high-fidelity tRNA decoding.

Ribosome*RelA structures reveal the mechanism of stringent response activation.,Loveland AB, Bah E, Madireddy R, Zhang Y, Brilot AF, Grigorieff N, Korostelev AA Elife. 2016 Jul 19;5. pii: e17029. doi: 10.7554/eLife.17029. PMID:27434674<ref>PMID:27434674</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5kpw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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[[Category: Escherichia coli K-12]]
[[Category: Large Structures]]
[[Category: Bah E]]
[[Category: Brilot AF]]
[[Category: Grigorieff N]]
[[Category: Korostelev AA]]
[[Category: Loveland AB]]
[[Category: Madireddy R]]
[[Category: Zhang Y]]