View source for 1ltj

[[Image:1ltj.gif|left|200px]]<br />
<applet load="1ltj" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1ltj, resolution 2.80&Aring;" />
'''Crystal Structure of Recombinant Human Fibrinogen Fragment D with the Peptide Ligands Gly-Pro-Arg-Pro-Amide and Gly-His-Arg-Pro-Amide'''<br />

==Overview==
We report two crystal structures, each at a resolution of 2.8 A, of, recombinant human fibrinogen fragment D (rfD) in the absence and presence, of peptide ligands. The bound ligands, Gly-Pro-Arg-Pro-amide and, Gly-His-Arg-Pro-amide, mimic the interactions of the thrombin exposed, polymerization sites, "A" and "B", respectively. This report is the first, to describe the structure of fragment D in the presence of both peptide, ligands. The structures reveal that recombinant fibrinogen is nearly, identical to the plasma protein but with minor changes, like the addition, of a proximal fucose to the carbohydrate linked to residue betaGln364, and, slightly different relative positions of the beta- and gamma-modules. Of, major interest in our structures is that a previously identified calcium, site in plasma fibrinogen is absent when Gly-His-Arg-Pro-amide is bound., The peptide-dependent loss of this calcium site may have significant, biological implications that are further discussed. These structures, provide a foundation for the detailed structural analysis of variant, recombinant fibrinogens that were used to identify critical functional, residues within fragment D.

==Disease==
Known diseases associated with this structure: Afibrinogenemia, congenital OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=134820 134820]], Afibrinogenemia, congenital OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=134830 134830]], Amyloidosis, hereditary renal OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=134820 134820]], Dysfibrinogenemia, alpha type, causing bleeding diathesis OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=134820 134820]], Dysfibrinogenemia, alpha type, causing recurrent thrombosis OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=134820 134820]], Dysfibrinogenemia, beta type OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=134830 134830]], Dysfibrinogenemia, gamma type OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=134850 134850]], Hypofibrinogenemia, gamma type OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=134850 134850]], Thrombophilia, dysfibrinogenemic OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=134830 134830]], Thrombophilia, dysfibrinogenemic OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=134850 134850]]

==About this Structure==
1LTJ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LTJ OCA]. 

==Reference==
2.8 A crystal structures of recombinant fibrinogen fragment D with and without two peptide ligands: GHRP binding to the "b" site disrupts its nearby calcium-binding site., Kostelansky MS, Betts L, Gorkun OV, Lord ST, Biochemistry. 2002 Oct 8;41(40):12124-32. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12356313 12356313]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Betts, L.]]
[[Category: Gorkun, O.V.]]
[[Category: Kostelansky, M.S.]]
[[Category: Lord, S.T.]]
[[Category: CA]]
[[Category: blood coagulation]]
[[Category: fibrinogen]]
[[Category: fibrinogen fragment d]]
[[Category: recombinant fibrinogen]]
[[Category: recombinant fibrinogen fragment d]]
[[Category: recombinant fibrinogen fragment d with two peptide ligands]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:04:08 2007''