View source for 1lcf

[[Image:1lcf.gif|left|200px]]<br />
<applet load="1lcf" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1lcf, resolution 2.0&Aring;" />
'''CRYSTAL STRUCTURE OF COPPER-AND OXALATE-SUBSTITUTED HUMAN LACTOFERRIN AT 2.0 ANGSTROMS RESOLUTION'''<br />

==Overview==
The three-dimensional structure of human dicupric monooxalate lactoferrin, Cu(2)oxLf, has been determined to 2.0 A resolution, using X-ray, diffraction data collected by diffractometry to 2.5 A resolution, and, oscillation photography on a synchrotron source to 2.0 A resolution., Difference electron-density maps calculated between Cu(2)oxLf and both, dicupric lactoferrin, Cu(2)Lf, and diferric lactoferrin, Fe(2)Lf, showed, that the oxalate had replaced a carbonate in the C-terminal binding site, and that, relative to Cu(2)Lf, there were no significant differences in, the N-terminal site. The structure was then refined crystallographically, by restrained least-squares methods. The final model, in which the r.m.s., deviation in bond distances is 0.017 A, contains 5314 protein atoms (691, residues), two Cu(2+) ions, one bicarbonate ion, one oxalate ion, 325, solvent molecules and one sugar residue. The crystallographic R factor of, 0.193 is for 46 134 reflections in the range 8.0 to 2.0 A resolution. The, oxalate ion is coordinated to copper in a 1,2-bidentate fashion, and the, added bulk of the anion results in the rearrangement of the side chains of, nearby arginine and tyrosine residues. No other major alterations in the, molecule can be observed, the overall protein structure being the same as, that for Cu(2)Lf and Fe(2)Lf.

==Disease==
Known disease associated with this structure: Deafness, autosomal dominant 1 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=602121 602121]]

==About this Structure==
1LCF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NAG, CU, CO3 and OXL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LCF OCA]. 

==Reference==
Structure of copper- and oxalate-substituted human lactoferrin at 2.0 A resolution., Smith CA, Anderson BF, Baker HM, Baker EN, Acta Crystallogr D Biol Crystallogr. 1994 May 1;50(Pt 3):302-16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299444 15299444]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Anderson, B.F.]]
[[Category: Baker, E.N.]]
[[Category: Baker, H.M.]]
[[Category: Smith, C.A.]]
[[Category: CO3]]
[[Category: CU]]
[[Category: NAG]]
[[Category: OXL]]
[[Category: iron transport]]

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