View source for 1lj2

[[Image:1lj2.gif|left|200px]]

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{{STRUCTURE_1lj2|  PDB=1lj2  |  SCENE=  }} 

'''Recognition of eIF4G by Rotavirus NSP3 reveals a basis for mRNA circularization'''


==Overview==
Rotaviruses, segmented double-stranded RNA viruses, co-opt the eukaryotic translation machinery with the aid of nonstructural protein 3 (NSP3), a rotaviral functional homolog of the cellular poly(A) binding protein (PABP). NSP3 binds to viral mRNA 3' consensus sequences and circularizes mRNA via interactions with eIF4G. Here, we present the X-ray structure of the C-terminal domain of NSP3 (NSP3-C) recognizing a fragment of eIF4GI. Homodimerization of NSP3-C yields a symmetric, elongated, largely alpha-helical structure with two hydrophobic eIF4G binding pockets at the dimer interface. Site-directed mutagenesis and isothermal titration calorimetry documented that NSP3 and PABP use analogous eIF4G recognition strategies, despite marked differences in tertiary structure.

==About this Structure==
1LJ2 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Simian_rotavirus_a/sa11 Simian rotavirus a/sa11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LJ2 OCA]. 

==Reference==
Recognition of eIF4G by rotavirus NSP3 reveals a basis for mRNA circularization., Groft CM, Burley SK, Mol Cell. 2002 Jun;9(6):1273-83. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12086624 12086624]
[[Category: Protein complex]]
[[Category: Simian rotavirus a/sa11]]
[[Category: Burley, S K.]]
[[Category: Groft, C M.]]
[[Category: Closed loop]]
[[Category: Coiled coil]]
[[Category: Eif4g]]
[[Category: Homodimer]]
[[Category: Mrna]]
[[Category: Nsp3]]
[[Category: Rotavirus]]
[[Category: Translation]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 23:58:01 2008''