View source for 1kpp

[[Image:1kpp.gif|left|200px]]<br />
<applet load="1kpp" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1kpp" />
'''Structure of the Tsg101 UEV domain'''<br />

==Overview==
Human Tsg101 plays key roles in HIV budding and in cellular vacuolar, protein sorting (VPS). In performing these functions, Tsg101 binds both, ubiquitin (Ub) and the PTAP tetrapeptide 'late domain' motif located, within the viral Gag protein. These interactions are mediated by the, N-terminal domain of Tsg101, which belongs to the catalytically inactive, ubiquitin E2 variant (UEV) family. We now report the structure of Tsg101, UEV and chemical shift mapping of the Ub and PTAP binding sites. Tsg101, UEV resembles canonical E2 ubiquitin conjugating enzymes, but has an, additional N-terminal helix, an extended beta-hairpin that links strands 1, and 2, and lacks the two C-terminal helices normally found in E2 enzymes., PTAP-containing peptides bind in a hydrophobic cleft exposed by the, absence of the C-terminal helices, whereas ubiquitin binds in a novel site, surrounding the beta-hairpin. These studies provide a structural framework, for understanding how Tsg101 mediates the protein-protein interactions, required for HIV budding and VPS.

==Disease==
Known disease associated with this structure: Breast cancer OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=601387 601387]]

==About this Structure==
1KPP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KPP OCA]. 

==Reference==
Structure and functional interactions of the Tsg101 UEV domain., Pornillos O, Alam SL, Rich RL, Myszka DG, Davis DR, Sundquist WI, EMBO J. 2002 May 15;21(10):2397-406. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12006492 12006492]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Alam, S.L.]]
[[Category: Davis, D.R.]]
[[Category: Myszka, D.G.]]
[[Category: Pornillos, O.]]
[[Category: Rich, R.L.]]
[[Category: Sundquist, W.I.]]
[[Category: e2 fold]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:53:24 2007''