View source for 4z7w


==T316 complex==
<StructureSection load='4z7w' size='340' side='right'caption='[[4z7w]], [[Resolution|resolution]] 2.89&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4z7w]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z7W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Z7W FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4z7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z7w OCA], [https://pdbe.org/4z7w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4z7w RCSB], [https://www.ebi.ac.uk/pdbsum/4z7w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4z7w ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q30069_HUMAN Q30069_HUMAN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In HLA-DQ8-associated celiac disease (CD), the pathogenic T cell response is directed toward an immunodominant alpha-gliadin-derived peptide (DQ8-glia-alpha1). However, our knowledge of TCR gene usage within the primary intestinal tissue of HLA-DQ8+ CD patients is limited. We identified two populations of HLA-DQ8-glia-alpha1 tetramer+ CD4+ T cells that were essentially undetectable in biopsy samples from patients on a gluten-free diet but expanded rapidly and specifically after antigenic stimulation. Distinguished by expression of TRBV9, both T cell populations displayed biased clonotypic repertoires and reacted similarly against HLA-DQ8-glia-alpha1. In particular, TRBV9 paired most often with TRAV26-2, whereas the majority of TRBV9- TCRs used TRBV6-1 with no clear TRAV gene preference. Strikingly, both tetramer+/TRBV9+ and tetramer+/TRBV9- T cells possessed a non-germline-encoded arginine residue in their CDR3alpha and CDR3beta loops, respectively. Comparison of the crystal structures of three TRBV9+ TCRs and a TRBV9- TCR revealed that, as a result of distinct TCR docking modes, the HLA-DQ8-glia-alpha1 contacts mediated by the CDR3-encoded arginine were almost identical between TRBV9+ and TRBV9- TCRs. In all cases, this interaction centered on two hydrogen bonds with a specific serine residue in the bound peptide. Replacement of serine with alanine at this position abrogated TRBV9+ and TRBV9- clonal T cell proliferation in response to HLA-DQ8-glia-alpha1. Gluten-specific memory CD4+ T cells with structurally and functionally conserved TCRs therefore predominate in the disease-affected tissue of patients with HLA-DQ8-mediated CD.

Determinants of Gliadin-Specific T Cell Selection in Celiac Disease.,Petersen J, van Bergen J, Loh KL, Kooy-Winkelaar Y, Beringer DX, Thompson A, Bakker SF, Mulder CJ, Ladell K, McLaren JE, Price DA, Rossjohn J, Reid HH, Koning F J Immunol. 2015 May 6. pii: 1500161. PMID:25948817<ref>PMID:25948817</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4z7w" style="background-color:#fffaf0;"></div>

==See Also==
*[[Gluten|Gluten]]
*[[T-cell receptor 3D structures|T-cell receptor 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Triticum aestivum]]
[[Category: Koning F]]
[[Category: Petersen J]]
[[Category: Reid HH]]
[[Category: Rossjohn J]]