View source for 4w29


==70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state.==
<StructureSection load='4w29' size='340' side='right'caption='[[4w29]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4w29]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB27 Thermus thermophilus HB27] and [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4qs0 4qs0], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4qs1 4qs1], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4qs2 4qs2] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4qs3 4qs3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4W29 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4W29 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=FUA:FUSIDIC+ACID'>FUA</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NMY:NEOMYCIN'>NMY</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4w29 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4w29 OCA], [https://pdbe.org/4w29 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4w29 RCSB], [https://www.ebi.ac.uk/pdbsum/4w29 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4w29 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RS3_THET2 RS3_THET2] Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Coupled translocation of messenger RNA and transfer RNA (tRNA) through the ribosome, a process catalyzed by elongation factor EF-G, is a crucial step in protein synthesis. The crystal structure of a bacterial translocation complex describes the binding states of two tRNAs trapped in mid-translocation. The deacylated P-site tRNA has moved into a partly translocated pe/E chimeric hybrid state. The anticodon stem-loop of the A-site tRNA is captured in transition toward the 30S P site, while its 3' acceptor end contacts both the A and P loops of the 50S subunit, forming an ap/ap chimeric hybrid state. The structure shows how features of ribosomal RNA rearrange to hand off the A-site tRNA to the P site, revealing an active role for ribosomal RNA in the translocation process.

How the ribosome hands the A-site tRNA to the P site during EF-G-catalyzed translocation.,Zhou J, Lancaster L, Donohue JP, Noller HF Science. 2014 Sep 5;345(6201):1188-91. doi: 10.1126/science.1255030. PMID:25190797<ref>PMID:25190797</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4w29" style="background-color:#fffaf0;"></div>

==See Also==
*[[Elongation factor 3D structures|Elongation factor 3D structures]]
*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermus thermophilus HB27]]
[[Category: Thermus thermophilus HB8]]
[[Category: Donohue JP]]
[[Category: Lancaster L]]
[[Category: Noller HF]]
[[Category: Zhou J]]