View source for 1iwt

[[Image:1iwt.gif|left|200px]]<br />
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'''Crystal Structure Analysis of Human lysozyme at 113K.'''<br />

==Overview==
The static and dynamic structures of human lysozyme at seven different, temperatures ranging from 113 to 178 K were investigated by normal-mode, refinement of the cryogenic X-ray diffraction data collected from a single, crystal. Normal-mode refinement decomposes the mean-square fluctuations of, protein atoms from their average position into the contributions from the, internal degrees of freedom, which change the shape of the protein, structure, and those from the external degrees of freedom, which generate, rigid-body motions in the crystal. While at temperatures below 150 K the, temperature dependence of the total mean-square fluctuations shows a small, gradient similar to that predicted theoretically by normal-mode analysis, at temperatures above 150 K there is an apparent inflection in the, temperature dependence with a higher gradient. The inflection in the, temperature dependence at temperatures above 150 K occurs mostly in the, external degrees of freedom. Possible causes for the dynamic transition, are discussed with respect to the crystal packing and physicochemical, properties of crystalline water.

==Disease==
Known diseases associated with this structure: Amyloidosis, renal OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=153450 153450]], Microphthalmia, syndromic 1 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=309800 309800]]

==About this Structure==
1IWT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CL as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IWT OCA]. 

==Reference==
Nonlinear temperature dependence of the crystal structure of lysozyme: correlation between coordinate shifts and thermal factors., Joti Y, Nakasako M, Kidera A, Go N, Acta Crystallogr D Biol Crystallogr. 2002 Sep;58(Pt 9):1421-32. Epub 2002, Aug 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12198298 12198298]
[[Category: Homo sapiens]]
[[Category: Lysozyme]]
[[Category: Single protein]]
[[Category: Go, N.]]
[[Category: Joti, Y.]]
[[Category: Kidera, A.]]
[[Category: Nakasako, M.]]
[[Category: CL]]
[[Category: glycosydase]]
[[Category: o-glycosyl]]

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