View source for 2m0u


==Complex structure of C-terminal CFTR peptide and extended PDZ1 domain from NHERF1==
<StructureSection load='2m0u' size='340' side='right'caption='[[2m0u]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2m0u]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M0U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M0U FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m0u OCA], [https://pdbe.org/2m0u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m0u RCSB], [https://www.ebi.ac.uk/pdbsum/2m0u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m0u ProSAT]</span></td></tr>
</table>
== Disease ==
[https://www.uniprot.org/uniprot/NHRF1_HUMAN NHRF1_HUMAN] Defects in SLC9A3R1 are the cause of hypophosphatemic nephrolithiasis/osteoporosis type 2 (NPHLOP2) [MIM:[https://omim.org/entry/612287 612287]. Hypophosphatemia results from idiopathic renal phosphate loss. It contributes to the pathogenesis of hypophosphatemic urolithiasis (formation of urinary calculi) as well to that of hypophosphatemic osteoporosis (bone demineralization).<ref>PMID:18784102</ref> <ref>PMID:22506049</ref> 
== Function ==
[https://www.uniprot.org/uniprot/NHRF1_HUMAN NHRF1_HUMAN] Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Involved in the regulation of phosphate reabsorption in the renal proximal tubules.<ref>PMID:9430655</ref> <ref>PMID:9096337</ref> <ref>PMID:10499588</ref> <ref>PMID:18784102</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The multi-domain scaffolding protein NHERF1 modulates the assembly and intracellular trafficking of various transmembrane receptors and ion-transport proteins. The two PDZ (postsynaptic density 95/disk large/zonula occluden 1) domains of NHERF1 possess very different ligand-binding capabilities: PDZ1 recognizes a variety of membrane proteins with high affinity, while PDZ2 only binds limited number of target proteins. Here using NMR, we have determined the structural and dynamic mechanisms that differentiate the binding affinities of the two PDZ domains, for the type 1 PDZ-binding motif (QDTRL) in the carboxyl terminus of cystic fibrosis transmembrane regulator. Similar to PDZ2, we have identified a helix-turn-helix subdomain coupled to the canonical PDZ1 domain. The extended PDZ1 domain is highly flexible with correlated backbone motions on fast and slow timescales, while the extended PDZ2 domain is relatively rigid. The malleability of the extended PDZ1 structure facilitates the transmission of conformational changes at the ligand-binding site to the remote helix-turn-helix extension. By contrast, ligand binding has only modest effects on the conformation and dynamics of the extended PDZ2 domain. The study shows that ligand-induced structural and dynamic changes coupled with sequence variation at the putative PDZ binding site dictate ligand selectivity and binding affinity of the two PDZ domains of NHERF1.

Ligand-Induced Dynamic Changes in Extended PDZ Domains from NHERF1.,Bhattacharya S, Ju JH, Orlova N, Khajeh JA, Cowburn D, Bu Z J Mol Biol. 2013 Apr 10. pii: S0022-2836(13)00209-X. doi:, 10.1016/j.jmb.2013.04.001. PMID:23583913<ref>PMID:23583913</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2m0u" style="background-color:#fffaf0;"></div>

==See Also==
*[[Sodium-hydrogen exchange regulatory factor|Sodium-hydrogen exchange regulatory factor]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Bhattacharya S]]
[[Category: Bu Z]]
[[Category: Cowburn D]]
[[Category: Ju JH]]