View source for 1jvx

[[Image:1jvx.gif|left|200px]]

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{{STRUCTURE_1jvx|  PDB=1jvx  |  SCENE=  }} 

'''Maltodextrin-binding protein variant D207C/A301GS/P316C cross-linked in crystal'''


==Overview==
Cysteine substitutions were engineered on the surface of maltose binding protein to produce crystine fibers, linear polymers of folded protein formed within a crystal. Disulfide bond formation between adjacent protein molecules within the lattice was monitored by X-ray crystallography. The cross-linked crystals were resistant to dissolution in water or neutral buffer solutions, even though the cross-linking was one-dimensional. However, crystine fibers were observed by transmission electron microscopy to dissociate from the crystals in acidic solutions. Some fibers remained associated as two-dimensional bundles or sheets, with a repeat unit along the fibers consistent with the packing of the individual protein molecules in the crystal. Neutralization of the acidic solutions caused the fibers to re-associate as a solid. Crystine threads were drawn out of this solution. In scanning electron microscopy images, many individual fibers could be seen unwinding from the ends of some threads. Crystine fibers are a new type of biomolecular material with potential applications wherever the use of proteins in a fibrous form is desirable, for example, the incorporation of enzymes into cloth or filtration material.

==About this Structure==
1JVX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JVX OCA]. 

==Reference==
Crystine: fibrous biomolecular material from protein crystals cross-linked in a specific geometry., Srinivasan U, Iyer GH, Przybycien TA, Samsonoff WA, Bell JA, Protein Eng. 2002 Nov;15(11):895-902. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12538909 12538909]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Bell, J A.]]
[[Category: Iyer, G H.]]
[[Category: Przybycien, T A.]]
[[Category: Samsonoff, W A.]]
[[Category: Srinivasan, U.]]
[[Category: Cross-link]]
[[Category: Disulfide]]
[[Category: Intermolecular]]
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