View source for 1jqs

[[Image:1jqs.gif|left|200px]]

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{{STRUCTURE_1jqs|  PDB=1jqs  |  SCENE=  }} 

'''Fitting of L11 protein and elongation factor G (domain G' and V) in the cryo-em map of E. coli 70S ribosome bound with EF-G and GMPPCP, a nonhydrolysable GTP analog'''


==Overview==
L11 protein is located at the base of the L7/L12 stalk of the 50 S subunit of the Escherichia coli ribosome. Because of the flexible nature of the region, recent X-ray crystallographic studies of the 50 S subunit failed to locate the N-terminal domain of the protein. We have determined the position of the complete L11 protein by comparing a three-dimensional cryo-EM reconstruction of the 70 S ribosome, isolated from a mutant lacking ribosomal protein L11, with the three-dimensional map of the wild-type ribosome. Fitting of the X-ray coordinates of L11-23 S RNA complex and EF-G into the cryo-EM maps combined with molecular modeling, reveals that, following EF-G-dependent GTP hydrolysis, domain V of EF-G intrudes into the cleft between the 23 S ribosomal RNA and the N-terminal domain of L11 (where the antibiotic thiostrepton binds), causing the N-terminal domain to move and thereby inducing the formation of the arc-like connection with the G' domain of EF-G. The results provide a new insight into the mechanism of EF-G-dependent translocation.

==About this Structure==
1JQS is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JQS OCA]. 

==Reference==
Localization of L11 protein on the ribosome and elucidation of its involvement in EF-G-dependent translocation., Agrawal RK, Linde J, Sengupta J, Nierhaus KH, Frank J, J Mol Biol. 2001 Aug 24;311(4):777-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11518530 11518530]
[[Category: Escherichia coli]]
[[Category: Protein complex]]
[[Category: Agrawal, R K.]]
[[Category: Frank, J.]]
[[Category: Linde, J.]]
[[Category: Nierhaus, K H.]]
[[Category: Segupta, J.]]
[[Category: 70s e coli ribosome]]
[[Category: Cryo-em]]
[[Category: Ef-g]]
[[Category: Gtp state]]
[[Category: L11]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 21:41:04 2008''