View source for 8ffr


==Revised structure of the rabies virus nucleoprotein-RNA complex==
<StructureSection load='8ffr' size='340' side='right'caption='[[8ffr]], [[Resolution|resolution]] 3.49&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8ffr]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Rabies_lyssavirus Rabies lyssavirus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FFR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FFR FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ffr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ffr OCA], [https://pdbe.org/8ffr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ffr RCSB], [https://www.ebi.ac.uk/pdbsum/8ffr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ffr ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A8VR20_9RHAB A8VR20_9RHAB] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
As for all non-segmented negative RNA viruses, rabies virus has its genome packaged in a linear assembly of nucleoprotein (N), named nucleocapsid. The formation of new nucleocapsids during virus replication in cells requires the production of soluble N protein in complex with its phosphoprotein (P) chaperone. In this study, we reconstituted a soluble heterodimeric complex between an armless N protein of rabies virus (RABV), lacking its N-terminal subdomain (N(NT-ARM)), and a peptide encompassing the N(0) chaperon module of the P protein. We showed that the chaperone module undergoes a disordered-order transition when it assembles with N(0) and measured an affinity in the low nanomolar range using a competition assay. We solved the crystal structure of the complex at a resolution of 2.3 A, unveiling the details of the conserved interfaces. MD simulations showed that both the chaperon module of P and RNA-mediated polymerization reduced the ability of the RNA binding cavity to open and close. Finally, by reconstituting a complex with full-length P protein, we demonstrated that each P dimer could independently chaperon two N(0) molecules.

Structure and Dynamics of the Unassembled Nucleoprotein of Rabies Virus in Complex with Its Phosphoprotein Chaperone Module.,Gerard FCA, Bourhis JM, Mas C, Branchard A, Vu DD, Varhoshkova S, Leyrat C, Jamin M Viruses. 2022 Dec 16;14(12):2813. doi: 10.3390/v14122813. PMID:36560817<ref>PMID:36560817</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8ffr" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Rabies lyssavirus]]
[[Category: Synthetic construct]]
[[Category: Albertini AAV]]
[[Category: Bourhis JM]]
[[Category: Jamin M]]
[[Category: Leyrat C]]
[[Category: Muziol T]]
[[Category: Ravelli RBG]]
[[Category: Ruigrok RWH]]
[[Category: Weissenhorn W]]
[[Category: Wernimont AK]]