View source for 1itz

[[Image:1itz.gif|left|200px]]

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{{STRUCTURE_1itz|  PDB=1itz  |  SCENE=  }} 

'''Maize Transketolase in complex with TPP'''


==Overview==
The gene specifying plastid transketolase (TK) of maize (Zea mays) was cloned from a cDNA library by southern blotting using a heterologous probe from sorghum (Sorghum bicolor). A recombinant fusion protein comprising thioredoxin of Escherichia coli and mature TK of maize was expressed at a high level in E. coli and cleaved with thrombin, affording plastid TK. The protein in complex with thiamine pyrophoshate was crystallized, and its structure was solved by molecular replacement. The enzyme is a C2 symmetric homodimer closely similar to the enzyme from yeast (Saccharomyces cerevisiae). Each subunit is folded into three domains. The two topologically equivalent active sites are located in the subunit interface region and resemble those of the yeast enzyme.

==About this Structure==
1ITZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ITZ OCA]. 

==Reference==
Structure and properties of an engineered transketolase from maize., Gerhardt S, Echt S, Busch M, Freigang J, Auerbach G, Bader G, Martin WF, Bacher A, Huber R, Fischer M, Plant Physiol. 2003 Aug;132(4):1941-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12913150 12913150]
[[Category: Single protein]]
[[Category: Transketolase]]
[[Category: Zea mays]]
[[Category: Bacher, A.]]
[[Category: Bader, G.]]
[[Category: Busch, M.]]
[[Category: Echt, S.]]
[[Category: Fischer, M.]]
[[Category: Freigang, J.]]
[[Category: Gerhardt, S.]]
[[Category: Huber, R.]]
[[Category: Calvin cycle]]
[[Category: Cofactor]]
[[Category: Plant]]
[[Category: Thiamine pyrophosphate]]
[[Category: Transketolase]]
[[Category: Zea may]]
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