View source for 1gzr

[[Image:1gzr.gif|left|200px]]<br />
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'''HUMAN INSULIN-LIKE GROWTH FACTOR; ESRF DATA'''<br />

==Overview==
Human insulin-like growth factors I and II (hIGF-I, hIGF-II) are potent, stimulators of cell and growth processes. They display high sequence, similarity to both the A and B chains of insulin but contain an additional, connecting C-domain, which reflects their secretion without specific, packaging or precursor conversion. IGFs also have an extension at the, C-terminus known as the D-domain. This paper describes four homologous, hIGF-1 structures, obtained from crystals grown in the presence of the, detergent SB12, which reveal additional detail in the C- and D-domains., Two different detergent binding modes observed in the crystals may reflect, different hIGF-I biological properties such as the interaction with IGF, binding proteins and self-aggregation. While the helical core of hIGF-I is, very similar to that in insulin, there are distinct differences in the, region of hIGF-I corresponding to the insulin B chain C-terminus, residues, B25-B30. In hIGF-I, these residues (24-29) and the following C-domain form, an extensive loop protruding 20 A from the core, which results in a, substantially different conformation for the receptor binding epitope in, hIGF-I compared to insulin. One notable feature of the structures, presented here is demonstration of peptide-bond cleavage between Ser35 and, Arg36 resulting in an apparent gap between residues 35 and 39. The, equivalent region of proinsulin is involved in hormone processing, demanding a reassessment of the structural integrity of hIGF-I in relation, to its biological function.

==Disease==
Known disease associated with this structure: Growth retardation with deafness and mental retardation due to IGF1 deficiency OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=147440 147440]]

==About this Structure==
1GZR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with C15 as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: C15. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GZR OCA]. 

==Reference==
Structural origins of the functional divergence of human insulin-like growth factor-I and insulin., Brzozowski AM, Dodson EJ, Dodson GG, Murshudov GN, Verma C, Turkenburg JP, de Bree FM, Dauter Z, Biochemistry. 2002 Jul 30;41(30):9389-97. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12135360 12135360]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Bree, F.M.De.]]
[[Category: Brzozowski, A.M.]]
[[Category: Dauter, Z.]]
[[Category: Dodson, E.J.]]
[[Category: Dodson, G.G.]]
[[Category: Murshudov, G.]]
[[Category: Turkenburg, J.P.]]
[[Category: Verma, C.]]
[[Category: C15]]
[[Category: growth factor]]
[[Category: igf-1]]
[[Category: insulin family]]
[[Category: plasma]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:10:38 2007''