View source for 1ghu

[[Image:1ghu.gif|left|200px]]<br />
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'''NMR SOLUTION STRUCTURE OF GROWTH FACTOR RECEPTOR-BOUND PROTEIN 2 (GRB2) SH2 DOMAIN, 24 STRUCTURES'''<br />

==Overview==
A family of NMR solution structures of the growth factor receptor-bound, protein 2 (Grb2) SH2 domain has been determined by heteronuclear, multidimensional NMR. Proton, nitrogen, and carbon chemical shift, assignments have been made for the SH2 domain of Grb2. Assignments were, made from a combination of homonuclear two-dimensional and 15N- and, 13C-edited three-dimensional spectra at pH 6.2 and 298 K., Structure-induced proton and carbon secondary shifts were calculated and, used to facilitate the spectral assignment process. NOE, scalar coupling, secondary chemical shift, and amide proton exchange data were used to, characterize the secondary structural elements and hydrogen-bonding, network in the Grb2 SH2 domain. The three-dimensional structure of the, Grb2 SH2 domain was calculated using 1112 restraints obtained from NOE, coupling constant, and amide proton exchange data. The rmsd for the 24, calculated structures to the mean structure of the Grb2 SH2 domain was, 0.75 A for backbone and 1.28 A for all heavy atoms. The three-dimensional, fold of the Grb2 SH2 domain is similar to that observed for other SH2, domains and consists of two alpha-helical segments and eight beta-strands, six strands that make up two contiguous antiparallel beta-sheets, and two, strands that form two short parallel beta-sheets. The structure of the, phosphotyrosine binding pocket of Grb2 is similar to that observed for, other SH2 domains. The hydrophobic binding pocket of Grb2 is similar to, that observed for Src with the exception that tryptophan 121 of Grb2, occupies part of the pY+3 binding pocket. Structural implications for the, Grb2 SH2 domain selectivity at the pY+2 and pY+3 sites are discussed.

==Disease==
Known diseases associated with this structure: Central hypoventilation syndrome, congenital OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=100790 100790]], Haddad syndrome OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=100790 100790]]

==About this Structure==
1GHU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GHU OCA]. 

==Reference==
Nuclear magnetic resonance solution structure of the growth factor receptor-bound protein 2 Src homology 2 domain., Thornton KH, Mueller WT, McConnell P, Zhu G, Saltiel AR, Thanabal V, Biochemistry. 1996 Sep 10;35(36):11852-64. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8794768 8794768]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Mcconnell, P.]]
[[Category: Mueller, W.T.]]
[[Category: Saltiel, A.R.]]
[[Category: Thanabal, V.]]
[[Category: Thornton, K.H.]]
[[Category: Zhu, G.]]
[[Category: grb2]]
[[Category: sh2]]
[[Category: src homology 2 domain]]

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