View source for 4bai


==Mycobacterium tuberculosis Chorismate synthase before exposure to 266 nm UV laser==
<StructureSection load='4bai' size='340' side='right'caption='[[4bai]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4bai]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BAI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BAI FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ztb|1ztb]], [[4baj|4baj]]</div></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Chorismate_synthase Chorismate synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.3.5 4.2.3.5] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bai FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bai OCA], [https://pdbe.org/4bai PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bai RCSB], [https://www.ebi.ac.uk/pdbsum/4bai PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bai ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Selenomethionine labeling is the most common technique used in protein crystallography to derivatize recombinant proteins for experimental phasing using anomalous scattering at tunable synchrotron beamlines. Recently, it has been shown that UV radiation depletes electron density of selenium atoms of selenomethionine residues and that UV radiation- damage induced phasing (equivalent to single isomorphous replacement) protocol can be applied to calculate experimental phases. Here we present the straightforward integration of a UV source with an in-house diffractometer. We show how this setup can extend the capabilities of a sealed tube X-ray generator and be used for experimental phasing of selenium-labeled proteins.

In-house UV radiation damage induced phasing of selenomethionine-labeled protein structures.,Pereira PJ, Royant A, Panjikar S, de Sanctis D J Struct Biol. 2012 Nov 20. pii: S1047-8477(12)00315-2. doi:, 10.1016/j.jsb.2012.11.003. PMID:23178456<ref>PMID:23178456</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4bai" style="background-color:#fffaf0;"></div>

==See Also==
*[[Chorismate synthase|Chorismate synthase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Chorismate synthase]]
[[Category: Large Structures]]
[[Category: Panjikar, S]]
[[Category: Pereira, P J.B]]
[[Category: Royant, A]]
[[Category: Sanctis, D de]]
[[Category: Lyase]]