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==Crystal structure, recombinant expression and mutagenesis studies of the bifunctional catalase-phenol oxidase from Scytalidium thermophilum==
<StructureSection load='4aum' size='340' side='right'caption='[[4aum]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4aum]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_16463 Atcc 16463]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AUM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AUM FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HDD:CIS-HEME+D+HYDROXYCHLORIN+GAMMA-SPIROLACTONE'>HDD</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4aue|4aue]], [[4aul|4aul]], [[4aun|4aun]], [[4b2y|4b2y]], [[4b31|4b31]], [[4b40|4b40]]</div></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Catalase Catalase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4aum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aum OCA], [https://pdbe.org/4aum PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4aum RCSB], [https://www.ebi.ac.uk/pdbsum/4aum PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4aum ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Scytalidium thermophilum produces a catalase with phenol oxidase activity (CATPO) that catalyses the decomposition of hydrogen peroxide into oxygen and water and also oxidizes various phenolic compounds. A codon-optimized catpo gene was cloned and expressed in Escherichia coli. The crystal structures of native and recombinant S. thermophilum CATPO and two variants, H82N and V123F, were determined at resolutions of 2.7, 1.4, 1.5 and 1.9 A, respectively. The structure of CATPO reveals a homotetramer with 698 residues per subunit and with strong structural similarity to Penicillium vitale catalase. The haem component is cis-hydroxychlorin gamma-spirolactone, which is rotated 180 degrees with respect to small-subunit catalases. The haem-binding pocket contains two highly conserved water molecules on the distal side. The H82N mutation resulted in conversion of the native d-type haem to a b-type haem. Kinetic studies of the H82N and V123F mutants indicate that both activities are likely to be associated with the haem centre and suggest that the secondary oxidase activity may be a general feature of catalases in the absence of hydrogen peroxide.

Structure, recombinant expression and mutagenesis studies of the catalase with oxidase activity from Scytalidium thermophilum.,Yuzugullu Y, Trinh CH, Smith MA, Pearson AR, Phillips SE, Sutay Kocabas D, Bakir U, Ogel ZB, McPherson MJ Acta Crystallogr D Biol Crystallogr. 2013 Mar;69(Pt 3):398-408. doi:, 10.1107/S0907444912049001. Epub 2013 Feb 16. PMID:23519415<ref>PMID:23519415</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 4aum" style="background-color:#fffaf0;"></div>

==See Also==
*[[Catalase 3D structures|Catalase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Atcc 16463]]
[[Category: Catalase]]
[[Category: Large Structures]]
[[Category: Bakir, U]]
[[Category: Kocabas, D Sutay]]
[[Category: McPherson, M J]]
[[Category: Ogel, Z B]]
[[Category: Pearson, A R]]
[[Category: Phillips, S E.V]]
[[Category: Smith, M A]]
[[Category: Trinh, C H]]
[[Category: Yuzugullu, Y]]
[[Category: Oxidoreductase]]
[[Category: Phenol oxidase]]