View source for 1ht1

[[Image:1ht1.gif|left|200px]]

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{{STRUCTURE_1ht1|  PDB=1ht1  |  SCENE=  }} 

'''Nucleotide-Dependent Conformational Changes in a Protease-Associated ATPase HslU'''


==Overview==
BACKGROUND: The bacterial heat shock locus ATPase HslU is an AAA(+) protein that has structures known in many nucleotide-free and -bound states. Nucleotide is required for the formation of the biologically active HslU hexameric assembly. The hexameric HslU ATPase binds the dodecameric HslV peptidase and forms an ATP-dependent HslVU protease. RESULTS: We have characterized four distinct HslU conformational states, going sequentially from open to closed: the empty, SO(4), ATP, and ADP states. The nucleotide binds at a cleft formed by an alpha/beta domain and an alpha-helical domain in HslU. The four HslU states differ by a rotation of the alpha-helical domain. This classification leads to a correction of nucleotide identity in one structure and reveals the ATP hydrolysis-dependent structural changes in the HslVU complex, including a ring rotation and a conformational change of the HslU C terminus. This leads to an amended protein unfolding-coupled translocation mechanism. CONCLUSIONS: The observed nucleotide-dependent conformational changes in HslU and their governing principles provide a framework for the mechanistic understanding of other AAA(+) proteins.

==About this Structure==
1HT1 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HT1 OCA]. 

==Reference==
Nucleotide-dependent conformational changes in a protease-associated ATPase HsIU., Wang J, Song JJ, Seong IS, Franklin MC, Kamtekar S, Eom SH, Chung CH, Structure. 2001 Nov;9(11):1107-16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11709174 11709174]
[[Category: Escherichia coli]]
[[Category: Protein complex]]
[[Category: Chung, C H.]]
[[Category: Eom, S H.]]
[[Category: Franklin, M C.]]
[[Category: Kamtekar, S.]]
[[Category: Seong, I S.]]
[[Category: Song, J J.]]
[[Category: Wang, J.]]
[[Category: Hslvu]]
[[Category: Peptidase-atpase complex]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 19:12:04 2008''