View source for 1fie

[[Image:1fie.gif|left|200px]]<br />
<applet load="1fie" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1fie, resolution 2.5&Aring;" />
'''RECOMBINANT HUMAN COAGULATION FACTOR XIII'''<br />

==Overview==
The three-dimensional structure of the recombinant human factor XIII a2, dimer after cleavage by thrombin has been determined by X-ray, crystallography. Factor XIII zymogen was treated with bovine, alpha-thrombin in the presence of 3 mM CaCl2, and the cleaved protein was, crystallized from Tris buffered at pH 6.5 using ethanol as the, precipitating agent. Refinement of the molecular model of thrombin-cleaved, factor XIII against diffraction data from 10.0 to 2.5 A resolution has, been carried out to give a crystallographic R factor of 18.2%. The, structure of thrombin-cleaved factor XIII is remarkably similar to that of, the zymogen: there are no large conformational changes in the protein and, the 37 residue amino terminus activation peptide remains associated with, the rest of the molecule. This work shows that the activation peptide, upon thrombin cleavage, has the same conformation and occupies the same, position with respect to the rest of the molecule as it does in the, zymogen structure.

==Disease==
Known disease associated with this structure: Factor XIIIA deficiency OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=134570 134570]]

==About this Structure==
1FIE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Protein-glutamine_gamma-glutamyltransferase Protein-glutamine gamma-glutamyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.2.13 2.3.2.13] Structure known Active Sites: CAT and CBT. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FIE OCA]. 

==Reference==
Structural evidence that the activation peptide is not released upon thrombin cleavage of factor XIII., Yee VC, Pedersen LC, Bishop PD, Stenkamp RE, Teller DC, Thromb Res. 1995 Jun 1;78(5):389-97. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7660355 7660355]
[[Category: Homo sapiens]]
[[Category: Protein-glutamine gamma-glutamyltransferase]]
[[Category: Single protein]]
[[Category: Teller, D.C.]]
[[Category: Yee, V.C.]]
[[Category: acyltransferase]]
[[Category: blood coagulation]]
[[Category: transferase]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:53:36 2007''