View source for 1hdo

[[Image:1hdo.jpg|left|200px]]

<!--
The line below this paragraph, containing "STRUCTURE_1hdo", creates the "Structure Box" on the page.
You may change the PDB parameter (which sets the PDB file loaded into the applet) 
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
or leave the SCENE parameter empty for the default display.
-->
{{STRUCTURE_1hdo|  PDB=1hdo  |  SCENE=  }} 

'''HUMAN BILIVERDIN IX BETA REDUCTASE: NADP COMPLEX'''


==Overview==
Biliverdin IXbeta reductase (BVR-B) catalyzes the pyridine nucleotide-dependent production of bilirubin-IXbeta, the major heme catabolite during early fetal development. BVR-B displays a preference for biliverdin isomers without propionates straddling the C10 position, in contrast to biliverdin IXalpha reductase (BVR-A), the major form of BVR in adult human liver. In addition to its tetrapyrrole clearance role in the fetus, BVR-B has flavin and ferric reductase activities in the adult. We have solved the structure of human BVR-B in complex with NADP+ at 1.15 A resolution. Human BVR-B is a monomer displaying an alpha/beta dinucleotide binding fold. The structures of ternary complexes with mesobiliverdin IValpha, biliverdin IXalpha, FMN and lumichrome show that human BVR-B has a single substrate binding site, to which substrates and inhibitors bind primarily through hydrophobic interactions, explaining its broad specificity. The reducible atom of both biliverdin and flavin substrates lies above the reactive C4 of the cofactor, an appropriate position for direct hydride transfer. BVR-B discriminates against the biliverdin IXalpha isomer through steric hindrance at the bilatriene side chain binding pockets. The structure also explains the enzyme's preference for NADP(H) and its B-face stereospecificity.

==About this Structure==
1HDO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HDO OCA]. 

==Reference==
Structure of human biliverdin IXbeta reductase, an early fetal bilirubin IXbeta producing enzyme., Pereira PJ, Macedo-Ribeiro S, Parraga A, Perez-Luque R, Cunningham O, Darcy K, Mantle TJ, Coll M, Nat Struct Biol. 2001 Mar;8(3):215-20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11224564 11224564]
[[Category: Biliverdin reductase]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Coll, M.]]
[[Category: Cunningham, O.]]
[[Category: Darcy, K.]]
[[Category: Macedo-Ribeiro, S.]]
[[Category: Mantle, T J.]]
[[Category: Parraga, A.]]
[[Category: Pereira, P J.B.]]
[[Category: Perez-Luque, R.]]
[[Category: Alpha/beta dinucleotide binding fold]]
[[Category: Biliverdin-ix beta reductase]]
[[Category: Diaphorase]]
[[Category: Flavin reductase]]
[[Category: Foetal metabolism]]
[[Category: Green haem binding protein]]
[[Category: Haem degradation]]
[[Category: Methaemoglobin reductase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 18:44:24 2008''