View source for 2adw


==Crystal structure of Echinomycin-(ACGTACGT)2 solved by SAD==
<StructureSection load='2adw' size='340' side='right'caption='[[2adw]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2adw]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_echinatus Streptomyces echinatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ADW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ADW FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=QUI:2-CARBOXYQUINOXALINE'>QUI</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DSN:D-SERINE'>DSN</scene>, <scene name='pdbligand=MVA:N-METHYLVALINE'>MVA</scene>, <scene name='pdbligand=N2C:N,S-DIMETHYLCYSTEINE'>N2C</scene>, <scene name='pdbligand=NCY:N-METHYLCYSTEINE'>NCY</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[185d|185d]], [[193d|193d]], [[1pfe|1pfe]], [[1vs2|1vs2]], [[1xvk|1xvk]], [[1xvn|1xvn]], [[1xvr|1xvr]], [[2da8|2da8]], [[3go3|3go3]]</div></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2adw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2adw OCA], [https://pdbe.org/2adw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2adw RCSB], [https://www.ebi.ac.uk/pdbsum/2adw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2adw ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A new crystal form was obtained for the complex between (ACGTACGT)2 and echinomycin and X-ray data were collected to 1.6 A. The structure was phased by the SAD method based on a single unexpected anomalous scatterer that could be identified as a mixture of nickel and zinc by measurements of the anomalous scattering at different wavelengths. This cation is coordinated by two guanines from two different duplexes and four water molecules. The structure resembles previously reported crystal structures of DNA-echinomycin complexes, except that three of the eight base pairs flanking the echinomycin bisintercalator sites have the Watson-Crick rather than the Hoogsteen configuration. Hoogsteen binding was found for the corresponding base pairs of the crystallographically independent duplex, indicating that the two configurations are very close in energy.

Serendipitous SAD phasing of an echinomycin-(ACGTACGT)2 bisintercalation complex.,Cuesta-Seijo JA, Weiss MS, Sheldrick GM Acta Crystallogr D Biol Crystallogr. 2006 Apr;62(Pt 4):417-24. Epub 2006, Mar 18. PMID:16552143<ref>PMID:16552143</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2adw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces echinatus]]
[[Category: Cuesta-Seijo, J A]]
[[Category: Sheldrick, G M]]
[[Category: Antibiotic]]
[[Category: Antitumor]]
[[Category: Bisintercalator]]
[[Category: Depsipeptide]]
[[Category: Dna-antibiotic complex]]
[[Category: Hoogsteen basepair]]
[[Category: Quinoxaline]]
[[Category: Thioacetal]]