View source for 3oqu


==Crystal structure of native abscisic acid receptor PYL9 with ABA==
<StructureSection load='3oqu' size='340' side='right'caption='[[3oqu]], [[Resolution|resolution]] 2.68&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3oqu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OQU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OQU FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A8S:(2Z,4E)-5-[(1S)-1-HYDROXY-2,6,6-TRIMETHYL-4-OXOCYCLOHEX-2-EN-1-YL]-3-METHYLPENTA-2,4-DIENOIC+ACID'>A8S</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3klx|3klx]], [[3kl1|3kl1]]</div></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PYL9, RCAR1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3oqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oqu OCA], [https://pdbe.org/3oqu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3oqu RCSB], [https://www.ebi.ac.uk/pdbsum/3oqu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3oqu ProSAT]</span></td></tr>
</table>
== Function ==
[[https://www.uniprot.org/uniprot/PYL9_ARATH PYL9_ARATH]] Receptor for abscisic acid (ABA) required for ABA-mediated responses such as stomatal closure and germination inhibition. Inhibits the activity of group-A protein phosphatases type 2C (PP2Cs) when activated by ABA. Confers enhanced sensitivity to ABA.<ref>PMID:19407143</ref>  
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== Publication Abstract from PubMed ==
The phytohormone abscisic acid ((+)-ABA) plays a key role in many processes. The biological and biochemical activities of unnatural (-)-ABA have been extensively investigated since 1960s. However, the recognition mechanism by which only a few members among PYR/PYL/RCAR (PYLs) family can bind (-)-ABA remains largely unknown. Here we systematically characterized the affinity of PYLs binding to the (-)-ABA and reported the crystal structures of apo-PYL5, PYL3-(-)-ABA and PYL9-(+)-ABA. PYL5 showed the strongest binding affinity with (-)-ABA among all the PYLs. PYL9 is a stringently exclusive (+)-ABA receptor with interchangeable disulfide bonds shared by a subclass of PYLs. PYL3 is a dual receptor to both ABA enantiomers. The binding orientation and pocket of (-)-ABA in PYLs are obviously different from those of (+)-ABA. Steric hindrance and hydrophobic interaction are the two key factors in determining the stereospecificity of PYLs binding to (-)-ABA, which is further confirmed by gain-of-function and loss-of-function mutagenesis. Our results provide novel insights of the bioactivity of ABA enantiomers onto PYLs, and shed light on designing the selective ABA receptors agonists.

Structural Insights into the Abscisic Acid Stereospecificity by the ABA Receptors PYR/PYL/RCAR.,Zhang X, Jiang L, Wang G, Yu L, Zhang Q, Xin Q, Wu W, Gong Z, Chen Z PLoS One. 2013 Jul 2;8(7):e67477. doi: 10.1371/journal.pone.0067477. Print 2013. PMID:23844015<ref>PMID:23844015</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3oqu" style="background-color:#fffaf0;"></div>

==See Also==
*[[Abscisic acid receptor 3D structures|Abscisic acid receptor 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Arath]]
[[Category: Large Structures]]
[[Category: Chen, Z]]
[[Category: Zhang, Q]]
[[Category: Zhang, X]]
[[Category: Abscisic acid receptor]]
[[Category: Hormone receptor]]
[[Category: Pyl9]]
[[Category: Rcar1]]