View source for 3lx2


==Crystal Structure analysis of PCNA from Thermococcus kodakaraensis tk0582==
<StructureSection load='3lx2' size='340' side='right'caption='[[3lx2]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3lx2]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/"thermococcus_kodakaraensis"_atomi_et_al._2004 "thermococcus kodakaraensis" atomi et al. 2004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LX2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LX2 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3lx1|3lx1]]</div></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pcn2, tk0535, TK0582 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=311400 "Thermococcus kodakaraensis" Atomi et al. 2004])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3lx2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lx2 OCA], [https://pdbe.org/3lx2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3lx2 RCSB], [https://www.ebi.ac.uk/pdbsum/3lx2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3lx2 ProSAT]</span></td></tr>
</table>
== Function ==
[[https://www.uniprot.org/uniprot/PCNA2_THEKO PCNA2_THEKO]] Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication.[HAMAP-Rule:MF_00317] 
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== Publication Abstract from PubMed ==
Proliferating cell nuclear antigen (PCNA) is a ring-shaped protein that encircles duplex DNA and plays an essential role in many DNA metabolic processes in archaea and eukarya. The eukaryotic and euryarchaea genomes contain a single gene encoding for PCNA. Interestingly, the genome of the euryarchaeon Thermococcus kodakaraensis contains two PCNA-encoding genes (TK0535 and TK0582), making it unique among the euryarchaea kingdom. It is shown here that the two T. kodakaraensis PCNA proteins support processive DNA synthesis by the polymerase. Both proteins form trimeric structures with characteristics similar to those of other archaeal and eukaryal PCNA proteins. One of the notable differences between the TK0535 and TK0582 rings is that the interfaces are different, resulting in different stabilities for the two trimers. The possible implications of these observations for PCNA functions are discussed.

Crystal structures of two active proliferating cell nuclear antigens (PCNAs) encoded by Thermococcus kodakaraensis.,Ladner JE, Pan M, Hurwitz J, Kelman Z Proc Natl Acad Sci U S A. 2011 Feb 15;108(7):2711-6. Epub 2011 Jan 26. PMID:21270332<ref>PMID:21270332</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3lx2" style="background-color:#fffaf0;"></div>

==See Also==
*[[Proliferating cell nuclear antigen 3D structures|Proliferating cell nuclear antigen 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Thermococcus kodakaraensis atomi et al. 2004]]
[[Category: Large Structures]]
[[Category: Kelman, Z]]
[[Category: Ladner, J E]]
[[Category: Pan, M]]
[[Category: Dna binding protein]]
[[Category: Dna processivity factor]]
[[Category: Dna replication]]
[[Category: Dna-binding]]
[[Category: Pcna]]
[[Category: Toroidal]]
[[Category: Trimer]]