View source for 2vy1


==Structure of LEAFY transcription factor from Arabidopsis thaliana in complex with DNA from AP1 promoter==
<StructureSection load='2vy1' size='340' side='right'caption='[[2vy1]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2vy1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VY1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VY1 FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2vy2|2vy2]]</div></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vy1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vy1 OCA], [https://pdbe.org/2vy1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vy1 RCSB], [https://www.ebi.ac.uk/pdbsum/2vy1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vy1 ProSAT]</span></td></tr>
</table>
== Function ==
[[https://www.uniprot.org/uniprot/LFY_ARATH LFY_ARATH]] Probable transcription factor that promotes early floral meristem identity in synergy with APETALA1. Is required subsequently for the transition of an inflorescence meristem into a floral meristem, by an immediate upstream regulation of the ABC classes of floral homeotic genes. Activates directly APETALA1, CAULIFLOWER and AGAMOUS, and indirectly APETALA3 and PISTILLATA with the cooperation of UFO.[REFERENCE:6]<ref>PMID:8565821</ref> <ref>PMID:9783581</ref>  
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vy/2vy1_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vy1 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The LEAFY (LFY) protein is a key regulator of flower development in angiosperms. Its gradually increased expression governs the sharp floral transition, and LFY subsequently controls the patterning of flower meristems by inducing the expression of floral homeotic genes. Despite a wealth of genetic data, how LFY functions at the molecular level is poorly understood. Here, we report crystal structures for the DNA-binding domain of Arabidopsis thaliana LFY bound to two target promoter elements. LFY adopts a novel seven-helix fold that binds DNA as a cooperative dimer, forming base-specific contacts in both the major and minor grooves. Cooperativity is mediated by two basic residues and plausibly accounts for LFY's effectiveness in triggering sharp developmental transitions. Our structure reveals an unexpected similarity between LFY and helix-turn-helix proteins, including homeodomain proteins known to regulate morphogenesis in higher eukaryotes. The appearance of flowering plants has been linked to the molecular evolution of LFY. Our study provides a unique framework to elucidate the molecular mechanisms underlying floral development and the evolutionary history of flowering plants.

Structural basis for LEAFY floral switch function and similarity with helix-turn-helix proteins.,Hames C, Ptchelkine D, Grimm C, Thevenon E, Moyroud E, Gerard F, Martiel JL, Benlloch R, Parcy F, Muller CW EMBO J. 2008 Oct 8;27(19):2628-37. Epub 2008 Sep 11. PMID:18784751<ref>PMID:18784751</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2vy1" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Arath]]
[[Category: Large Structures]]
[[Category: Benlloch, R]]
[[Category: Gerard, F]]
[[Category: Grimm, C]]
[[Category: Hames, C]]
[[Category: Martiel, J L]]
[[Category: Moyroud, E]]
[[Category: Muller, C W]]
[[Category: Parcy, F]]
[[Category: Ptchelkine, D]]
[[Category: Thevenon, E]]
[[Category: Activator]]
[[Category: Coiled coil]]
[[Category: Developmental protein]]
[[Category: Differentiation]]
[[Category: Dna-binding]]
[[Category: Flower development]]
[[Category: Flowering]]
[[Category: Homeotic gene]]
[[Category: Nucleus]]
[[Category: Polymorphism]]
[[Category: Transcription]]
[[Category: Transcription regulation]]