View source for 1gaw

[[Image:1gaw.gif|left|200px]]

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{{STRUCTURE_1gaw|  PDB=1gaw  |  SCENE=  }} 

'''CRYSTAL STRUCTURE ANALYSIS OF THE FERREDOXIN-NADP+ REDUCTASE FROM MAIZE LEAF'''


==Overview==
All oxygenic photosynthetically derived reducing equivalents are utilized by combinations of a single multifuctional electron carrier protein, ferredoxin (Fd), and several Fd-dependent oxidoreductases. We report the first crystal structure of the complex between maize leaf Fd and Fd-NADP(+) oxidoreductase (FNR). The redox centers in the complex--the 2Fe-2S cluster of Fd and flavin adenine dinucleotide (FAD) of FNR--are in close proximity; the shortest distance is 6.0 A. The intermolecular interactions in the complex are mainly electrostatic, occurring through salt bridges, and the interface near the prosthetic groups is hydrophobic. NMR experiments on the complex in solution confirmed the FNR recognition sites on Fd that are identified in the crystal structure. Interestingly, the structures of Fd and FNR in the complex and in the free state differ in several ways. For example, in the active site of FNR, Fd binding induces the formation of a new hydrogen bond between side chains of Glu 312 and Ser 96 of FNR. We propose that this type of molecular communication not only determines the optimal orientation of the two proteins for electron transfer, but also contributes to the modulation of the enzymatic properties of FNR.

==About this Structure==
1GAW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GAW OCA]. 

==Reference==
Structure of the electron transfer complex between ferredoxin and ferredoxin-NADP(+) reductase., Kurisu G, Kusunoki M, Katoh E, Yamazaki T, Teshima K, Onda Y, Kimata-Ariga Y, Hase T, Nat Struct Biol. 2001 Feb;8(2):117-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11175898 11175898]
[[Category: Single protein]]
[[Category: Zea mays]]
[[Category: Hase, T.]]
[[Category: Kurisu, G.]]
[[Category: Kusunoki, M.]]
[[Category: Oxidoreductase/electron transport]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 17:21:44 2008''