View source for 2bs1


==MS2 (N87AE89K mutant) - Qbeta RNA hairpin complex==
<StructureSection load='2bs1' size='340' side='right'caption='[[2bs1]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2bs1]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteriophage_ms2 Bacteriophage ms2] and [https://en.wikipedia.org/wiki/Bacteriophage_ms2 Bacteriophage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BS1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BS1 FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1aq3|1aq3]], [[1aq4|1aq4]], [[1bms|1bms]], [[1dzs|1dzs]], [[1gkv|1gkv]], [[1gkw|1gkw]], [[1kuo|1kuo]], [[1msc|1msc]], [[1mst|1mst]], [[1mva|1mva]], [[1mvb|1mvb]], [[1u1y|1u1y]], [[1zdh|1zdh]], [[1zdi|1zdi]], [[1zdj|1zdj]], [[1zdk|1zdk]], [[2bny|2bny]], [[2bq5|2bq5]], [[2bs0|2bs0]], [[2bu1|2bu1]], [[2c4q|2c4q]], [[2c4y|2c4y]], [[2c4z|2c4z]], [[2c50|2c50]], [[2c51|2c51]], [[2ms2|2ms2]], [[5msf|5msf]], [[6msf|6msf]], [[7msf|7msf]]</div></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bs1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bs1 OCA], [https://pdbe.org/2bs1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bs1 RCSB], [https://www.ebi.ac.uk/pdbsum/2bs1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bs1 ProSAT]</span></td></tr>
</table>
== Function ==
[[https://www.uniprot.org/uniprot/COAT_BPMS2 COAT_BPMS2]] Forms the phage shell; binds to the phage RNA. 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bs/2bs1_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bs1 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Sequence-specific interactions between RNA stem-loops and coat protein (CP) subunits play vital roles in the life cycles of the RNA bacteriophages, e.g., by allowing translational repression of their replicase cistrons and tagging their own RNA genomes for encapsidation. The CPs of bacteriophages Qbeta and MS2 each discriminate in favor of their cognate translational operators, even in the presence of closely related operators from other phages in vivo. Discrete mutations within the MS2 CP have been shown to relax this discrimination in vitro. We have determined the structures of eight complexes between such mutants and both MS2 and Qbeta stem-loops with X-ray crystallography. In conjunction with previously determined in vivo repression data, the structures enable us to propose the molecular basis for the discrimination mechanism.

Structural basis of RNA binding discrimination between bacteriophages Qbeta and MS2.,Horn WT, Tars K, Grahn E, Helgstrand C, Baron AJ, Lago H, Adams CJ, Peabody DS, Phillips SE, Stonehouse NJ, Liljas L, Stockley PG Structure. 2006 Mar;14(3):487-95. PMID:16531233<ref>PMID:16531233</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2bs1" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacteriophage ms2]]
[[Category: Large Structures]]
[[Category: Adams, C J]]
[[Category: Baron, A J]]
[[Category: Grahn, E]]
[[Category: Helgstrand, C]]
[[Category: Horn, W T]]
[[Category: Lago, H]]
[[Category: Liljas, L]]
[[Category: Peabody, D S]]
[[Category: Phillips, S E.V]]
[[Category: Stockley, P G]]
[[Category: Stonehouse, N J]]
[[Category: Tars, K]]
[[Category: Capsid]]
[[Category: Hairpin]]
[[Category: Icosahedral virus]]
[[Category: Levivirus]]
[[Category: Virus-rna complex]]
[[Category: Virus/rna]]
[[Category: Virus/viral protein/rna]]