View source for 1qvs


==Crystal Structure of Haemophilus influenzae H9A mutant Holo Ferric ion-Binding Protein A==
<StructureSection load='1qvs' size='340' side='right'caption='[[1qvs]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1qvs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacterium_influenzae"_lehmann_and_neumann_1896 "bacterium influenzae" lehmann and neumann 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QVS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QVS FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FBPA, HITA, FBP or HI0097 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 "Bacterium influenzae" Lehmann and Neumann 1896])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qvs OCA], [https://pdbe.org/1qvs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qvs RCSB], [https://www.ebi.ac.uk/pdbsum/1qvs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qvs ProSAT]</span></td></tr>
</table>
== Function ==
[[https://www.uniprot.org/uniprot/FBPA_HAEIN FBPA_HAEIN]] Part of the ABC transporter complex FbpABC (TC 3.A.1.10.1) involved in Fe(3+) ions import. This protein specifically binds Fe(3+) and is involved in its transmembrane transport (By similarity). 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qv/1qvs_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qvs ConSurf].
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== Publication Abstract from PubMed ==
The periplasmic iron binding protein plays an essential role in the iron uptake pathway of Gram-negative pathogenic bacteria from the Pasteurellaceae and Neisseriaceae families and is critical for survival of these pathogens within the host. In this study, we report the crystal structures of two mutant forms of ferric ion-binding protein A (FbpA) from Haemophilus influenzae with bound multinuclear oxo-metal clusters. Crystals of site-directed mutants in the metal or anion binding ligands contain protein in the open conformation, and two mutant FbpAs, H9A and N175L, contain different cluster arrangements in the iron-binding pocket. The iron clusters are anchored by binding to the two tyrosine ligands (Tyr195 and Tyr196) positioned at the vertex of the iron-binding pocket but are not coordinated by the other metal binding ligands. Our results suggest that the metal clusters may have formed in situ, suggesting that the mutant FbpAs may serve as a simple model for protein-mediated mineralization.

Presence of ferric hydroxide clusters in mutants of Haemophilus influenzae ferric ion-binding protein A.,Shouldice SR, Skene RJ, Dougan DR, McRee DE, Tari LW, Schryvers AB Biochemistry. 2003 Oct 21;42(41):11908-14. PMID:14556621<ref>PMID:14556621</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 1qvs" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ferric-binding protein|Ferric-binding protein]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacterium influenzae lehmann and neumann 1896]]
[[Category: Large Structures]]
[[Category: Dougan, D R]]
[[Category: McRee, D E]]
[[Category: Schryvers, A B]]
[[Category: Shouldice, S R]]
[[Category: Skene, R J]]
[[Category: Tari, L W]]
[[Category: Metal binding protein]]