View source for 3dqh


==Structure of the Yellow Fluorescent Protein Citrine Frozen at 1000 Atmospheres Number 5: Structure 9 in a Series of 26 High Pressure Structures==
<StructureSection load='3dqh' size='340' side='right'caption='[[3dqh]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3dqh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DQH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DQH FirstGlance]. <br>
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1huy|1huy]], [[1yfp|1yfp]], [[2yfp|2yfp]], [[1f0b|1f0b]], [[1f09|1f09]], [[3dpw|3dpw]], [[3dpx|3dpx]], [[3dpz|3dpz]], [[3dq1|3dq1]], [[3dq2|3dq2]], [[3dq3|3dq3]], [[3dq4|3dq4]], [[3dq5|3dq5]], [[3dq6|3dq6]], [[3dq7|3dq7]], [[3dq8|3dq8]], [[3dq9|3dq9]], [[3dqa|3dqa]], [[3dqc|3dqc]], [[3dqd|3dqd]], [[3dqe|3dqe]], [[3dqf|3dqf]], [[3dqi|3dqi]], [[3dqj|3dqj]], [[3dqk|3dqk]], [[3dql|3dql]], [[3dqm|3dqm]], [[3dqn|3dqn]], [[3dqo|3dqo]], [[3dqu|3dqu]]</div></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GFP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dqh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dqh OCA], [https://pdbe.org/3dqh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dqh RCSB], [https://www.ebi.ac.uk/pdbsum/3dqh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dqh ProSAT]</span></td></tr>
</table>
== Function ==
[[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dq/3dqh_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3dqh ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A protein molecule is an intricate system whose function is highly sensitive to small external perturbations. However, no examples that correlate protein function with progressive subangstrom structural perturbations have thus far been presented. To elucidate this relationship, we have investigated a fluorescent protein, citrine, as a model system under high-pressure perturbation. The protein has been compressed to produce deformations of its chromophore by applying a high-pressure cryocooling technique. A closely spaced series of x-ray crystallographic structures reveals that the chromophore undergoes a progressive deformation of up to 0.8 A at an applied pressure of 500 MPa. It is experimentally demonstrated that the structural motion is directly correlated with the progressive fluorescence shift of citrine from yellow to green under these conditions. This protein is therefore highly sensitive to subangstrom deformations and its function must be understood at the subangstrom level. These results have significant implications for protein function prediction and biomolecule design and engineering, because they suggest methods to tune protein function by modification of the protein scaffold.

Alteration of citrine structure by hydrostatic pressure explains the accompanying spectral shift.,Barstow B, Ando N, Kim CU, Gruner SM Proc Natl Acad Sci U S A. 2008 Sep 9;105(36):13362-6. Epub 2008 Sep 3. PMID:18768811<ref>PMID:18768811</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3dqh" style="background-color:#fffaf0;"></div>

==See Also==
*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Aeqvi]]
[[Category: Large Structures]]
[[Category: Barstow, B]]
[[Category: Kim, C U]]
[[Category: Beta barrel]]
[[Category: Chromophore]]
[[Category: Fluorescent protein]]
[[Category: High pressure]]
[[Category: Luminescence]]
[[Category: Luminescent protein]]
[[Category: Photoprotein]]
[[Category: Yellow fluorescent protein]]