View source for 2osq


==NMR Structure of RRM-1 of Yeast NPL3 Protein==
<StructureSection load='2osq' size='340' side='right'caption='[[2osq]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2osq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OSQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OSQ FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2osr|2osr]]</div></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NOP3, MTS1, NAB1, NPL3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2osq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2osq OCA], [https://pdbe.org/2osq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2osq RCSB], [https://www.ebi.ac.uk/pdbsum/2osq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2osq ProSAT]</span></td></tr>
</table>
== Function ==
[[https://www.uniprot.org/uniprot/NOP3_YEAST NOP3_YEAST]] Required for pre-rRNA processing and nuclear import as well as mitochondrial protein targeting. Binds to poly(A). 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/os/2osq_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2osq ConSurf].
<div style="clear:both"></div>
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== Publication Abstract from PubMed ==
Yeast Npl3 is homologous to SR proteins in higher eukaryotes, a family of RNA-binding proteins that have multiple essential roles in RNA metabolism. This protein competes with 3'-end processing factors for binding to the nascent RNA, protecting the transcript from premature termination and coordinating transcription termination and the packaging of the fully processed transcript for export. The NMR structure of its RNA-binding domain shows two unusually compact RNA recognition motifs (RRMs), and identifies the RNA recognition surface in Npl3. Biochemical and NMR studies identify a class of G+U-rich RNA sequences with high specificity for this protein. The protein binds to RNA and forms a single globular structure, but the two RRMs of Npl3 are not equivalent, with the second domain forming much stronger interactions with G+U-rich RNA sequences that occur independently of the interaction of the first RRM. The specific binding to G+U-rich RNAs observed for the two RRMs of Npl3 is masked in the full-length protein by a much stronger but non-sequence-specific RNA-binding activity residing outside of its RRMs. The preference of Npl3 for G+U-rich sequences supports the model for its function in regulating recognition of 3'-end processing sites through competition with the Rna15 (yeast analog of human CstF-64 protein) subunit of the processing complex.

Structure of the yeast SR protein Npl3 and Interaction with mRNA 3'-end processing signals.,Deka P, Bucheli ME, Moore C, Buratowski S, Varani G J Mol Biol. 2008 Jan 4;375(1):136-50. Epub 2007 Sep 16. PMID:18022637<ref>PMID:18022637</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Atcc 18824]]
[[Category: Large Structures]]
[[Category: Allain, F H]]
[[Category: Bucheli, M]]
[[Category: Buratowski, S]]
[[Category: Deka, P]]
[[Category: Moore, C]]
[[Category: Skrisovska, L]]
[[Category: Varani, G]]
[[Category: Mrna]]
[[Category: Npl3]]
[[Category: Rna binding protein]]
[[Category: Rna-binding]]
[[Category: Rrm]]
[[Category: Sr protein]]