View source for 2ae4


==Glutaryl 7-Aminocephalosporanic Acid Acylase: mutational study of activation mechanism==
<StructureSection load='2ae4' size='340' side='right'caption='[[2ae4]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2ae4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._gk-16 Pseudomonas sp. gk-16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AE4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AE4 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2adv|2adv]], [[2ae3|2ae3]], [[2ae5|2ae5]]</div></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ae4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ae4 OCA], [https://pdbe.org/2ae4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ae4 RCSB], [https://www.ebi.ac.uk/pdbsum/2ae4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ae4 ProSAT]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ae/2ae4_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ae4 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cephalosporin acylase (CA), a member of the N-terminal nucleophile hydrolase family, is activated through sequential primary and secondary autoproteolytic reactions with the release of a pro segment. We have determined crystal structures of four CA mutants. Two mutants are trapped after the primary cleavage, and the other two undergo secondary cleavage slowly. These structures provide a look at pro-segment conformation during activation in N-terminal nucleophile hydrolases. The highly strained helical pro segment of precursor is transformed into a relaxed loop in the intermediates, suggesting that the relaxation of structural constraints drives the primary cleavage reaction. The secondary autoproteolytic step has been proposed to be intermolecular. However, our analysis provides evidence that CA is processed in two sequential steps of intramolecular autoproteolysis involving two distinct residues in the active site, the first a serine and the second a glutamate.

Insight into autoproteolytic activation from the structure of cephalosporin acylase: a protein with two proteolytic chemistries.,Kim JK, Yang IS, Shin HJ, Cho KJ, Ryu EK, Kim SH, Park SS, Kim KH Proc Natl Acad Sci U S A. 2006 Feb 7;103(6):1732-7. Epub 2006 Jan 30. PMID:16446446<ref>PMID:16446446</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2ae4" style="background-color:#fffaf0;"></div>

==See Also==
*[[Cephalosporin acylase 3D structures|Cephalosporin acylase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Penicillin amidase]]
[[Category: Pseudomonas sp. gk-16]]
[[Category: Cho, K J]]
[[Category: Kim, J K]]
[[Category: Kim, K H]]
[[Category: Kim, S H]]
[[Category: Park, S S]]
[[Category: Ryu, E K]]
[[Category: Shin, H J]]
[[Category: Yang, I S]]
[[Category: Autoproteolysis]]
[[Category: Cephalosporin acylase]]
[[Category: Hydrolase]]
[[Category: Intermediate structure]]
[[Category: Precursor activation]]