View source for 2vb1


==HEWL at 0.65 angstrom resolution==
<StructureSection load='2vb1' size='340' side='right'caption='[[2vb1]], [[Resolution|resolution]] 0.65&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2vb1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VB1 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2VB1 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1aki|1aki]], [[1azf|1azf]], [[1b2k|1b2k]], [[1bgi|1bgi]], [[1bvx|1bvx]], [[1bwj|1bwj]], [[1c10|1c10]], [[1dpw|1dpw]], [[1dqj|1dqj]], [[1f0w|1f0w]], [[1f10|1f10]], [[1fdl|1fdl]], [[1flq|1flq]], [[1flu|1flu]], [[1flw|1flw]], [[1fn5|1fn5]], [[1g7i|1g7i]], [[1g7l|1g7l]], [[1gwd|1gwd]], [[1gxv|1gxv]], [[1h6m|1h6m]], [[1hc0|1hc0]], [[1hel|1hel]], [[1hen|1hen]], [[1heo|1heo]], [[132l|132l]], [[193l|193l]], [[194l|194l]], [[1a2y|1a2y]], [[1at5|1at5]], [[1at6|1at6]], [[1b0d|1b0d]], [[1bhz|1bhz]], [[1bvk|1bvk]], [[1bwh|1bwh]], [[1bwi|1bwi]], [[1c08|1c08]], [[1dpx|1dpx]], [[1e8l|1e8l]], [[1f3j|1f3j]], [[1fly|1fly]], [[1g7h|1g7h]], [[1g7j|1g7j]], [[1g7m|1g7m]], [[1gpq|1gpq]], [[1gxx|1gxx]], [[1h87|1h87]], [[1hem|1hem]], [[1heq|1heq]], [[1her|1her]], [[1hsw|1hsw]], [[1hep|1hep]], [[1hew|1hew]], [[1hf4|1hf4]], [[1ic4|1ic4]], [[1ic5|1ic5]], [[1ic7|1ic7]], [[1iee|1iee]], [[1io5|1io5]], [[1ioq|1ioq]], [[1ios|1ios]], [[1iot|1iot]], [[1ir7|1ir7]], [[1ir9|1ir9]], [[1j1x|1j1x]], [[1ja2|1ja2]], [[1ja6|1ja6]], [[1jis|1jis]], [[1jit|1jit]], [[1jj0|1jj0]], [[1jj1|1jj1]], [[1jj3|1jj3]], [[1kip|1kip]], [[1kir|1kir]], [[1lcn|1lcn]], [[1hsx|1hsx]], [[1ior|1ior]], [[1ir8|1ir8]], [[1j1o|1j1o]], [[1j1p|1j1p]], [[1ja4|1ja4]], [[1ja7|1ja7]], [[1jiy|1jiy]], [[1jpo|1jpo]], [[1jto|1jto]], [[1jtt|1jtt]], [[1kiq|1kiq]], [[1kxw|1kxw]], [[1kxx|1kxx]], [[1kxy|1kxy]], [[1lj3|1lj3]], [[1lj4|1lj4]], [[1ljf|1ljf]], [[1ljh|1ljh]], [[1ljj|1ljj]], [[1ljk|1ljk]], [[1lkr|1lkr]], [[1lks|1lks]], [[1lpi|1lpi]], [[1lsa|1lsa]], [[1lse|1lse]], [[1lje|1lje]], [[1ljg|1ljg]], [[1lji|1lji]], [[1lma|1lma]], [[1lsb|1lsb]], [[1lsc|1lsc]], [[1lsd|1lsd]], [[1lsf|1lsf]], [[1lsm|1lsm]], [[1lsn|1lsn]], [[1lsy|1lsy]], [[1lys|1lys]], [[1lz9|1lz9]], [[1lzc|1lzc]], [[1lze|1lze]], [[1lzg|1lzg]], [[1lzh|1lzh]], [[1mel|1mel]], [[1n4f|1n4f]], [[1nby|1nby]], [[1ndg|1ndg]], [[1qio|1qio]], [[1ri8|1ri8]], [[1rjc|1rjc]], [[1sf6|1sf6]], [[1sfg|1sfg]], [[1lsg|1lsg]], [[1lsz|1lsz]], [[1lyo|1lyo]], [[1lyz|1lyz]], [[1lz8|1lz8]], [[1lza|1lza]], [[1lzb|1lzb]], [[1lzd|1lzd]], [[1lzn|1lzn]], [[1lzt|1lzt]], [[1mlc|1mlc]], [[1nbz|1nbz]], [[1ndm|1ndm]], [[1p2c|1p2c]], [[1ps5|1ps5]], [[1qtk|1qtk]], [[1rcm|1rcm]], [[1rfp|1rfp]], [[1sf4|1sf4]], [[1sf7|1sf7]], [[1sfb|1sfb]], [[1sq2|1sq2]], [[1t3p|1t3p]], [[1t6v|1t6v]], [[1ua6|1ua6]], [[1uc0|1uc0]], [[1uco|1uco]], [[1uib|1uib]], [[1uif|1uif]], [[1vat|1vat]], [[1vdp|1vdp]], [[1vds|1vds]], [[1vdt|1vdt]], [[1ved|1ved]], [[1vfb|1vfb]], [[1w6z|1w6z]], [[1wtm|1wtm]], [[1wtn|1wtn]], [[1xej|1xej]], [[1xgq|1xgq]], [[1yik|1yik]], [[1ykx|1ykx]], [[1yl0|1yl0]], [[1yqv|1yqv]], [[1zmy|1zmy]], [[2aub|2aub]], [[2bly|2bly]], [[2bpu|2bpu]], [[1uia|1uia]], [[1uic|1uic]], [[1uid|1uid]], [[1uie|1uie]], [[1uig|1uig]], [[1uih|1uih]], [[1uuz|1uuz]], [[1v7s|1v7s]], [[1v7t|1v7t]], [[1vau|1vau]], [[1vdq|1vdq]], [[1xei|1xei]], [[1xek|1xek]], [[1xfp|1xfp]], [[1xgp|1xgp]], [[1yil|1yil]], [[1yky|1yky]], [[1ykz|1ykz]], [[1yl1|1yl1]], [[1z55|1z55]], [[2a6u|2a6u]], [[2a7d|2a7d]], [[2a7f|2a7f]], [[2b5z|2b5z]], [[2blx|2blx]], [[2c8o|2c8o]], [[2c8p|2c8p]], [[2cds|2cds]], [[2d4i|2d4i]], [[2d6b|2d6b]], [[2d91|2d91]], [[2lyo|2lyo]], [[3hfm|3hfm]], [[3lyt|3lyt]], [[3lyz|3lyz]], [[4lyo|4lyo]], [[5lyz|5lyz]], [[6lyt|6lyt]], [[6lyz|6lyz]], [[7lyz|7lyz]], [[8lyz|8lyz]], [[2cgi|2cgi]], [[2d4j|2d4j]], [[2d4k|2d4k]], [[2fbb|2fbb]], [[2hfm|2hfm]], [[2iff|2iff]], [[2lym|2lym]], [[2lyz|2lyz]], [[2lzh|2lzh]], [[2lzt|2lzt]], [[3lym|3lym]], [[3lyo|3lyo]], [[3lzt|3lzt]], [[4lym|4lym]], [[4lyt|4lyt]], [[4lyz|4lyz]], [[4lzt|4lzt]], [[5lym|5lym]], [[5lyt|5lyt]]</div></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2vb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vb1 OCA], [http://pdbe.org/2vb1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vb1 RCSB], [http://www.ebi.ac.uk/pdbsum/2vb1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vb1 ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>  
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vb/2vb1_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vb1 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of triclinic hen egg-white lysozyme (HEWL) has been refined against diffraction data extending to 0.65 A resolution measured at 100 K using synchrotron radiation. Refinement with anisotropic displacement parameters and with the removal of stereochemical restraints for the well ordered parts of the structure converged with a conventional R factor of 8.39% and an R(free) of 9.52%. The use of full-matrix refinement provided an estimate of the variances in the derived parameters. In addition to the 129-residue protein, a total of 170 water molecules, nine nitrate ions, one acetate ion and three ethylene glycol molecules were located in the electron-density map. Eight sections of the main chain and many side chains were modeled with alternate conformations. The occupancies of the water sites were refined and this step is meaningful when assessed by use of the free R factor. A detailed description and comparison of the structure are made with reference to the previously reported triclinic HEWL structures refined at 0.925 A (at the low temperature of 120 K) and at 0.95 A resolution (at room temperature).

Triclinic lysozyme at 0.65 A resolution.,Wang J, Dauter M, Alkire R, Joachimiak A, Dauter Z Acta Crystallogr D Biol Crystallogr. 2007 Dec;63(Pt 12):1254-68. Epub 2007, Nov 16. PMID:18084073<ref>PMID:18084073</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2vb1" style="background-color:#fffaf0;"></div>

==See Also==
*[[Lysozyme 3D structures|Lysozyme 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Gallus gallus]]
[[Category: Large Structures]]
[[Category: Lysozyme]]
[[Category: Alkire, R]]
[[Category: Dauter, M]]
[[Category: Dauter, Z]]
[[Category: Joachimiak, A]]
[[Category: Wang, J]]
[[Category: Allergen]]
[[Category: Antimicrobial]]
[[Category: Atomic resolution]]
[[Category: Bacteriolytic enzyme]]
[[Category: Glycosidase]]
[[Category: Hydrolase]]
[[Category: Triclinic hewl]]