View source for 5y2h


==Crystal structure of the oligomerization domain of NSP4 from the rotavirus strain MF66==
<StructureSection load='5y2h' size='340' side='right'caption='[[5y2h]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5y2h]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovine_rotavirus_g10 Bovine rotavirus g10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y2H OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5Y2H FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5y2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y2h OCA], [http://pdbe.org/5y2h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y2h RCSB], [http://www.ebi.ac.uk/pdbsum/5y2h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y2h ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Rotavirus nonstructural protein 4, the first viral enterotoxin to be identified, is a multidomain, multifunctional glycoprotein. Earlier, we reported a Ca(2+)-bound coiled-coil tetrameric structure of the diarrhea-inducing region of NSP4 from the rotavirus strains SA11 and I321 and a Ca(2+)-free pentameric structure from the rotavirus strain ST3, all with a parallel arrangement of alpha-helices. pH was found to determine the oligomeric state: a basic pH favoured a tetramer, whereas an acidic pH favoured a pentamer. Here, we report two novel forms of the coiled-coil region of NSP4 from the bovine rotavirus strains MF66 and NCDV. These crystallized at acidic pH, forming antiparallel coiled-coil tetrameric structures without any bound Ca(2+) ion. Structural and mutational studies of the coiled-coil regions of NSP4 revealed that the nature of the residue at position 131 (Tyr/His) plays an important role in the observed structural diversity.

New tetrameric forms of the rotavirus NSP4 with antiparallel helices.,Kumar S, Ramappa R, Pamidimukkala K, Rao CD, Suguna K Arch Virol. 2018 Feb 17. pii: 10.1007/s00705-018-3753-6. doi:, 10.1007/s00705-018-3753-6. PMID:29455326<ref>PMID:29455326</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
*[[Nonstructural protein 3D structures|Nonstructural protein 3D structures]]
== References ==
<references/>
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</StructureSection>
[[Category: Bovine rotavirus g10]]
[[Category: Large Structures]]
[[Category: Kumar, S]]
[[Category: Suguna, K]]
[[Category: Antiparallel tetramer nsp4 rotavirus coiled-coil mf66]]
[[Category: Viral protein]]