View source for 2xgi


==Crystal structure of Barley Beta-Amylase complexed with 3,4- epoxybutyl alpha-D-glucopyranoside==
<StructureSection load='2xgi' size='340' side='right'caption='[[2xgi]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2xgi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XGI OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2XGI FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EBQ:2-[(2R)-OXIRAN-2-YL]ETHYL+ALPHA-D-GLUCOPYRANOSIDE'>EBQ</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=J5B:(2~{R},3~{S},4~{S},5~{R},6~{S})-2-(hydroxymethyl)-6-[(3~{R})-3-oxidanylbutoxy]oxane-3,4,5-triol'>J5B</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2xg9|2xg9]], [[2xfy|2xfy]], [[2xgb|2xgb]], [[2xff|2xff]], [[1b1y|1b1y]], [[2xfr|2xfr]]</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-amylase Beta-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.2 3.2.1.2] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2xgi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xgi OCA], [http://pdbe.org/2xgi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xgi RCSB], [http://www.ebi.ac.uk/pdbsum/2xgi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xgi ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
There are major issues regarding the proposed pathway for starch degradation in germinating cereal grain. Given the commercial importance but genetic intractability of the problem, we have embarked on a program of chemical genetics studies to identify and dissect the pathway and regulation of starch degradation in germinating barley grains. As a precursor to in vivo studies, here we report systematic analysis of the reversible and irreversible inhibition of the major beta-amylase of the grain endosperm (BMY1). The molecular basis of inhibitor action was defined through high resolution X-ray crystallography studies of unliganded barley beta-amylase, as well as its complexes with glycone site binder disaccharide iminosugar G1M, irreversible inhibitors alpha-epoxypropyl and alpha-epoxybutyl glucosides, which target the enzyme's catalytic residues, and the aglycone site binders acarbose and alpha-cyclodextrin.

Chemical genetics and cereal starch metabolism: structural basis of the non-covalent and covalent inhibition of barley beta-amylase.,Rejzek M, Stevenson CE, Southard AM, Stanley D, Denyer K, Smith AM, Naldrett MJ, Lawson DM, Field RA Mol Biosyst. 2010 Nov 18. PMID:21085740<ref>PMID:21085740</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2xgi" style="background-color:#fffaf0;"></div>

==See Also==
*[[Amylase 3D structures|Amylase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Beta-amylase]]
[[Category: Hordeum vulgare]]
[[Category: Large Structures]]
[[Category: Denyer, K]]
[[Category: Field, R A]]
[[Category: Lawson, D M]]
[[Category: Naldrett, M J]]
[[Category: Rejzek, M]]
[[Category: Smith, A M]]
[[Category: Southard, A M]]
[[Category: Stanley, D]]
[[Category: Stevenson, C E.M]]
[[Category: Carbohydrate metabolism]]
[[Category: Germination]]
[[Category: Glycosidase]]
[[Category: Glycosyl hydrolase family 14]]
[[Category: Hydrolase]]
[[Category: Starch degradation]]