View source for 1bg5

[[Image:1bg5.gif|left|200px]]

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{{STRUCTURE_1bg5|  PDB=1bg5  |  SCENE=  }} 

'''CRYSTAL STRUCTURE OF THE ANKYRIN BINDING DOMAIN OF ALPHA-NA,K-ATPASE AS A FUSION PROTEIN WITH GLUTATHIONE S-TRANSFERASE'''


==Overview==
The ankyrin 33-residue repeating motif, an L-shaped structure with protruding beta-hairpin tips, mediates specific macromolecular interactions with cytoskeletal, membrane, and regulatory proteins. The association between ankyrin and alpha-Na,K-ATPase, a ubiquitous membrane protein critical to vectorial transport of ions and nutrients, is required to assemble and stabilize Na,K-ATPase at the plasma membrane. alpha-Na,K-ATPase binds both red cell ankyrin (AnkR, a product of the ANK1 gene) and Madin-Darby canine kidney cell ankyrin (AnkG, a product of the ANK3 gene) utilizing residues 142-166 (SYYQEAKSSKIMESFK NMVPQQALV) in its second cytoplasmic domain. Fusion peptides of glutathione S-transferase incorporating these 25 amino acids bind specifically to purified ankyrin (Kd = 118 +/- 50 nM). The three-dimensional structure (2.6 A) of this minimal ankyrin-binding motif, crystallized as the fusion protein, reveals a 7-residue loop with one charged hydrophilic face capping a double beta-strand. Comparison with ankyrin-binding sequences in p53, CD44, neurofascin/L1, and the inositol 1,4,5-trisphosphate receptor suggests that the valency and specificity of ankyrin binding is achieved by the interaction of 5-7-residue surface loops with the beta-hairpin tips of multiple ankyrin repeat units.

==About this Structure==
1BG5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BG5 OCA]. 

==Reference==
Structure of the ankyrin-binding domain of alpha-Na,K-ATPase., Zhang Z, Devarajan P, Dorfman AL, Morrow JS, J Biol Chem. 1998 Jul 24;273(30):18681-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9668035 9668035]
[[Category: Rattus norvegicus]]
[[Category: Single protein]]
[[Category: Devarajan, P.]]
[[Category: Morrow, J S.]]
[[Category: Zhang, Z.]]
[[Category: Ankyrin binding]]
[[Category: Atpase]]
[[Category: Carrier crystallization]]
[[Category: Glutathione-s-transferase]]
[[Category: Ion transport]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 11:28:19 2008''